The Gárdos Channel and Piezo1 Revisited: Comparison between Reticulocytes and Mature Red Blood Cells.

Autor: Petkova-Kirova P; Institute of Neurobiology, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria.; Department of Biochemistry, Saarland University, 66123 Saarbrücken, Germany., Murciano N; Nanion Technologies, 80339 Munich, Germany.; Theoretical Medicine and Biosciences, Campus University Hospital, Saarland University, 66421 Homburg, Germany., Iacono G; Department of Hematopoiesis, Sanquin Research, 1066 CX Amsterdam, The Netherlands.; Landsteiner Laboratory, Amsterdam UMC, University of Amsterdam, 1007 MB Amsterdam, The Netherlands., Jansen J; Theoretical Medicine and Biosciences, Campus University Hospital, Saarland University, 66421 Homburg, Germany.; Department of Experimental Physics, Saarland University, 66123 Saarbrücken, Germany., Simionato G; Department of Experimental Physics, Saarland University, 66123 Saarbrücken, Germany.; Department of Experimental Surgery, Campus University Hospital, Saarland University, 66421 Homburg, Germany., Qiao M; Theoretical Medicine and Biosciences, Campus University Hospital, Saarland University, 66421 Homburg, Germany.; Department of Experimental Physics, Saarland University, 66123 Saarbrücken, Germany., Van der Zwaan C; Department of Hematopoiesis, Sanquin Research, 1066 CX Amsterdam, The Netherlands.; Landsteiner Laboratory, Amsterdam UMC, University of Amsterdam, 1007 MB Amsterdam, The Netherlands., Rotordam MG; Nanion Technologies, 80339 Munich, Germany., John T; Department of Experimental Physics, Saarland University, 66123 Saarbrücken, Germany., Hertz L; Theoretical Medicine and Biosciences, Campus University Hospital, Saarland University, 66421 Homburg, Germany.; Department of Experimental Physics, Saarland University, 66123 Saarbrücken, Germany., Hoogendijk AJ; Department of Hematopoiesis, Sanquin Research, 1066 CX Amsterdam, The Netherlands.; Landsteiner Laboratory, Amsterdam UMC, University of Amsterdam, 1007 MB Amsterdam, The Netherlands., Becker N; Nanion Technologies, 80339 Munich, Germany., Wagner C; Department of Experimental Physics, Saarland University, 66123 Saarbrücken, Germany.; Physics and Materials Science Research Unit, University of Luxembourg, L-1511 Luxembourg, Luxembourg., Von Lindern M; Department of Hematopoiesis, Sanquin Research, 1066 CX Amsterdam, The Netherlands.; Landsteiner Laboratory, Amsterdam UMC, University of Amsterdam, 1007 MB Amsterdam, The Netherlands., Egee S; Biological Station Roscoff, Sorbonne University, CNRS, UMR8227 LBI2M, F-29680 Roscoff, France.; Laboratory of Excellence GR-Ex, F-75015 Paris, France., Van den Akker E; Department of Hematopoiesis, Sanquin Research, 1066 CX Amsterdam, The Netherlands.; Landsteiner Laboratory, Amsterdam UMC, University of Amsterdam, 1007 MB Amsterdam, The Netherlands., Kaestner L; Theoretical Medicine and Biosciences, Campus University Hospital, Saarland University, 66421 Homburg, Germany.; Department of Experimental Physics, Saarland University, 66123 Saarbrücken, Germany.
Jazyk: angličtina
Zdroj: International journal of molecular sciences [Int J Mol Sci] 2024 Jan 24; Vol. 25 (3). Date of Electronic Publication: 2024 Jan 24.
DOI: 10.3390/ijms25031416
Abstrakt: The Gárdos channel (KCNN4) and Piezo1 are the best-known ion channels in the red blood cell (RBC) membrane. Nevertheless, the quantitative electrophysiological behavior of RBCs and its heterogeneity are still not completely understood. Here, we use state-of-the-art biochemical methods to probe for the abundance of the channels in RBCs. Furthermore, we utilize automated patch clamp, based on planar chips, to compare the activity of the two channels in reticulocytes and mature RBCs. In addition to this characterization, we performed membrane potential measurements to demonstrate the effect of channel activity and interplay on the RBC properties. Both the Gárdos channel and Piezo1, albeit their average copy number of activatable channels per cell is in the single-digit range, can be detected through transcriptome analysis of reticulocytes. Proteomics analysis of reticulocytes and mature RBCs could only detect Piezo1 but not the Gárdos channel. Furthermore, they can be reliably measured in the whole-cell configuration of the patch clamp method. While for the Gárdos channel, the activity in terms of ion currents is higher in reticulocytes compared to mature RBCs, for Piezo1, the tendency is the opposite. While the interplay between Piezo1 and Gárdos channel cannot be followed using the patch clamp measurements, it could be proved based on membrane potential measurements in populations of intact RBCs. We discuss the Gárdos channel and Piezo1 abundance, interdependencies and interactions in the context of their proposed physiological and pathophysiological functions, which are the passing of small constrictions, e.g., in the spleen, and their active participation in blood clot formation and thrombosis.
Databáze: MEDLINE
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