Enhancement of Female Rat Fertility via Ethanolic Extract from Nigella sativa L. (Black Cumin) Seeds Assessed via HPLC-ESI-MS/MS and Molecular Docking.

Autor: Nagy AM; Department of Animal Reproduction & AI, Veterinary Research Institute, National Research Center, Cairo 12622, Egypt., Abdelhameed MF; Department of Pharmacology, National Research Center, Cairo 12622, Egypt., Elkarim ASA; Chemistry of Tanning Materials and Leather Technology Department, National Research Center, Cairo 12622, Egypt., Sarker TC; Texas A&M AgriLife Research Center, Overton, TX 75684, USA., Abd-ElGawad AM; Plant Production Department, College of Food & Agriculture Sciences, King Saud University, P.O. Box 2460, Riyadh 11451, Saudi Arabia., Elshamy AI; Department of Natural Compounds Chemistry, National Research Center, Cairo 12622, Egypt., Hammam AM; Department of Animal Reproduction & AI, Veterinary Research Institute, National Research Center, Cairo 12622, Egypt.
Jazyk: angličtina
Zdroj: Molecules (Basel, Switzerland) [Molecules] 2024 Feb 05; Vol. 29 (3). Date of Electronic Publication: 2024 Feb 05.
DOI: 10.3390/molecules29030735
Abstrakt: The characteristic chemical composition of Nigella seeds is directly linked to their beneficial properties. This study aimed to investigate the phytochemical composition of Nigella sativa seeds using a 100% ethanolic extract using HPLC-ESI-MS/MS. Additionally, it explored the potential biological effects of the extract on female rat reproduction. Follicle Stimulating Hormone (FSH), Luteinizing Hormone (LH), Estrogen (E2), and Progesterone (P4) hormone levels were also assessed, along with the morphological and histological effects of the extract on ovarian, oviductal, and uterine tissues. Molecular docking was performed to understand the extract's activity and its role in regulating female reproduction by assessing its binding affinity to hormonal receptors. Twenty metabolites, including alkaloids, saponins, terpenes, flavonoids, phenolic acids, and fatty acids, were found in the ethanolic extract of N. sativa seeds through the HPLC-ESI-MS/MS study. The N. sativa seed extract exhibited strong estrogenic and LH-like activities ( p < 0.05) with weak FSH-like activity. Furthermore, it increased the serum levels of LH ( p < 0.05), P4 hormones ( p < 0.001), and E2 ( p < 0.0001). Molecular docking results displayed a strong interaction with Erβ, LH, GnRH, and P4 receptors, respectively. Based on these findings, N. sativa seeds demonstrated hormone-like activities, suggesting their potential as a treatment for improving female fertility.
Databáze: MEDLINE
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