Proteomics Analysis of Pregnancy in Ewes under Heat Stress Conditions and Melatonin Administration.

Autor: Bouroutzika E; Faculty of Veterinary Science, University of Thessaly, 43131 Karditsa, Greece., Proikakis S; Laboratory of Food Quality Control and Hygiene, Department of Food Science and Human Nutrition, Agricultural University of Athens, 11855 Athens, Greece., Theodosiadou EK; Faculty of Veterinary Science, University of Thessaly, 43131 Karditsa, Greece., Vougas K; Proteomics Research Unit, Biomedical Research Foundation of the Academy of Athens, 11527 Athens, Greece., Katsafadou AI; Faculty of Public and One Health, University of Thessaly, 43100 Karditsa, Greece., Tsangaris GT; Proteomics Research Unit, Biomedical Research Foundation of the Academy of Athens, 11527 Athens, Greece., Valasi I; Faculty of Veterinary Science, University of Thessaly, 43131 Karditsa, Greece.
Jazyk: angličtina
Zdroj: Animals : an open access journal from MDPI [Animals (Basel)] 2024 Jan 26; Vol. 14 (3). Date of Electronic Publication: 2024 Jan 26.
DOI: 10.3390/ani14030400
Abstrakt: Melatonin is an indoleamine with broad spectrum properties that acts as a regulator of antioxidant and immune response in organisms. In our previous studies, melatonin improved redox status and inflammatory response in pregnant ewes under heat stress conditions. In the present study, using proteomics, the proteins regulated by melatonin during different stages of pregnancy and lambing were assessed. Twenty-two ewes equally divided into two groups, the melatonin (M) (n = 11) and control (C) group (n = 11), participated in the study and were exposed to heat stress during the first months of pregnancy. In the M group, melatonin implants were administered throughout pregnancy, every 40 days, until parturition (a total of four implants per ewe). Blood samples were collected at the beginning of the study simultaneously with the administration of the first melatonin implant (blood samples M1, C1), mating (M2, C2), second implant (M3, C3), fourth implant (M4, C4) and parturition (M5, C5), and MALDI-TOF analysis was performed. The results revealed the existence of 42 extra proteins in samples M2, M3 and M4 and 53 in M5 (sample at parturition) that are linked to melatonin. The biological processes of these proteins refer to boosted immune response, the alleviation of oxidative and endoplasmic reticulum stress, energy metabolism, the protection of the maternal organism and embryo development. This proteomics analysis indicates that melatonin regulates protective mechanisms and controls cell proliferation under exogenous or endogenous stressful stimuli during pregnancy and parturition.
Databáze: MEDLINE
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