Otu and Rif1 Double Mutant Enables Analysis of Satellite DNA in Polytene Chromosomes of Ovarian Germ Cells in Drosophila melanogaster.

Autor: Kolesnikova TD; Institute of Molecular and Cellular Biology of the Siberian Branch of the RAS, Novosibirsk, Russia. doliolida@gmail.com., Nokhova AR; Novosibirsk State University, Novosibirsk, Russia., Shatskikh AS; National Research Centre 'Kurchatov Institute', Moscow, Russia., Klenov MS; Institute of Molecular Genetics RAS, Moscow, Russia., Zhimulev IF; Institute of Molecular and Cellular Biology of the Siberian Branch of the RAS, Novosibirsk, Russia.
Jazyk: angličtina
Zdroj: Doklady. Biochemistry and biophysics [Dokl Biochem Biophys] 2023 Dec; Vol. 513 (Suppl 1), pp. S87-S91. Date of Electronic Publication: 2024 Feb 09.
DOI: 10.1134/S160767292360046X
Abstrakt: Polytene chromosomes in Drosophila serve as a classical model for cytogenetic studies. However, heterochromatic regions of chromosomes are typically under-replicated, hindering their analysis. Mutations in the Rif1 gene lead to additional replication of heterochromatic sequences, including satellite DNA, in salivary gland cells. Here, we investigated the impact of the Rif1 mutation on heterochromatin in polytene chromosomes formed in ovarian germ cells due to the otu gene mutation. By the analysis of otu 11 ; Rif1 1 double mutants, we found that, in the presence of the Rif1 mutation, ovarian cells undergo additional polytenization of pericentromeric regions. This includes the formation of large chromatin blocks composed of satellite DNA. Thus, the effects of the Rif1 mutation are similar in salivary gland and germ cells. The otu 11 ; Rif1 1 system opens new possibilities for studying factors associated with heterochromatin during oogenesis.
(© 2023. Pleiades Publishing, Ltd.)
Databáze: MEDLINE
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