TALE-based organellar genome editing and gene expression in plants.

Autor: Lin JY; Agricultural Biotechnology Research Center, Academia Sinica, Tainan, 71150, Taiwan.; Department of Biotechnology and Bioindustry Sciences, National Cheng Kung University, Tainan, 70101, Taiwan., Liu YC; Agricultural Biotechnology Research Center, Academia Sinica, Tainan, 71150, Taiwan., Tseng YH; Department of Biotechnology and Bioindustry Sciences, National Cheng Kung University, Tainan, 70101, Taiwan., Chan MT; Agricultural Biotechnology Research Center, Academia Sinica, Tainan, 71150, Taiwan. mbmtchan@gate.sinica.edu.tw.; Department of Biotechnology and Bioindustry Sciences, National Cheng Kung University, Tainan, 70101, Taiwan. mbmtchan@gate.sinica.edu.tw., Chang CC; Department of Biotechnology and Bioindustry Sciences, National Cheng Kung University, Tainan, 70101, Taiwan. chingcc@mail.ncku.edu.tw.
Jazyk: angličtina
Zdroj: Plant cell reports [Plant Cell Rep] 2024 Feb 10; Vol. 43 (3), pp. 61. Date of Electronic Publication: 2024 Feb 10.
DOI: 10.1007/s00299-024-03150-w
Abstrakt: Key Message: TALE-based editors provide an alternative way to engineer the organellar genomes in plants. We update and discuss the most recent developments of TALE-based organellar genome editing in plants. Gene editing tools have been widely used to modify the nuclear genomes of plants for various basic research and biotechnological applications. The clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 editing platform is the most commonly used technique because of its ease of use, fast speed, and low cost; however, it encounters difficulty when being delivered to plant organelles for gene editing. In contrast, protein-based editing technologies, such as transcription activator-like effector (TALE)-based tools, could be easily delivered, expressed, and targeted to organelles in plants via Agrobacteria-mediated nuclear transformation. Therefore, TALE-based editors provide an alternative way to engineer the organellar genomes in plants since the conventional chloroplast transformation method encounters technical challenges and is limited to certain species, and the direct transformation of mitochondria in higher plants is not yet possible. In this review, we update and discuss the most recent developments of TALE-based organellar genome editing in plants.
(© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Databáze: MEDLINE
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