Basophils are important for development of allergic skin inflammation.

Autor: Leyva-Castillo JM; Division of Immunology, Boston Children's Hospital, and the Department of Pediatrics, Harvard Medical School, Boston, Mass. Electronic address: Manuel.LeyvaCastillo@childrens.harvard.edu., Vega-Mendoza D; Division of Immunology, Boston Children's Hospital, and the Department of Pediatrics, Harvard Medical School, Boston, Mass., Strakosha M; Division of Immunology, Boston Children's Hospital, and the Department of Pediatrics, Harvard Medical School, Boston, Mass., Deng L; Department of Immunology, Harvard Medical School, Boston, Mass., Choi S; Department of Immunology, Harvard Medical School, Boston, Mass., Miyake K; Inflammation, Infection and Immunity Laboratory, Advanced Research Institute, Tokyo Medical and Dental University, Tokyo, Japan., Karasuyama H; Inflammation, Infection and Immunity Laboratory, Advanced Research Institute, Tokyo Medical and Dental University, Tokyo, Japan., Chiu IM; Department of Immunology, Harvard Medical School, Boston, Mass., Phipatanakul W; Division of Immunology, Boston Children's Hospital, and the Department of Pediatrics, Harvard Medical School, Boston, Mass., Geha RS; Division of Immunology, Boston Children's Hospital, and the Department of Pediatrics, Harvard Medical School, Boston, Mass.
Jazyk: angličtina
Zdroj: The Journal of allergy and clinical immunology [J Allergy Clin Immunol] 2024 May; Vol. 153 (5), pp. 1344-1354.e5. Date of Electronic Publication: 2024 Feb 07.
DOI: 10.1016/j.jaci.2024.01.022
Abstrakt: Background: Atopic dermatitis skin lesions exhibit increased infiltration by basophils. Basophils produce IL-4, which plays an important role in the pathogenesis of atopic dermatitis.
Objective: We sought to determine the role of basophils in a mouse model of antigen-driven allergic skin inflammation.
Methods: Wild-type mice, mice with selective and inducible depletion of basophils, and mice expressing Il4-driven enhanced green fluorescent protein were subjected to epicutaneous sensitization with ovalbumin or saline. Sensitized skin was examined by histology for epidermal thickening. Cells were analyzed for surface markers and intracellular expression of enhanced green fluorescent protein by flow cytometry. Gene expression was evaluated by real-time reverse transcription-quantitative PCR.
Results: Basophils were important for epidermal hyperplasia, dermal infiltration by CD4 + T cells, mast cells, and eosinophils in ovalbumin-sensitized mouse skin and for the local and systemic T H 2 response to epicutaneous sensitization. Moreover, basophils were the major source of IL-4 in epicutaneous-sensitized mouse skin and promote the ability of dendritic cells to drive T H 2 polarization of naive T cells.
Conclusion: Basophils play an important role in the development of allergic skin inflammation induced by cutaneous exposure to antigen in mice.
(Copyright © 2024 American Academy of Allergy, Asthma & Immunology. Published by Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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