Magnetic capture device for large volume sample analysis.

Autor: Armstrong CM; United States Department of Agriculture, Agriculture Research Service, Eastern Regional Research Center, Wyndmoor, Pennsylvania, United States of America., Capobianco JA; United States Department of Agriculture, Agriculture Research Service, Eastern Regional Research Center, Wyndmoor, Pennsylvania, United States of America., Lee J; United States Department of Agriculture, Agriculture Research Service, Eastern Regional Research Center, Wyndmoor, Pennsylvania, United States of America.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2024 Feb 09; Vol. 19 (2), pp. e0297806. Date of Electronic Publication: 2024 Feb 09 (Print Publication: 2024).
DOI: 10.1371/journal.pone.0297806
Abstrakt: Immunomagnetic separation (IMS) techniques employing superparamagnetic particles can successfully isolate various components from mixtures. However, their utility can be limited for large-volume samples, viscous samples, or those containing a high density of particulate matter because of the need to generate high field gradients for particle recovery. Therefore, a new class of immunomagnetic particles was devised utilizing a single, macroscopic Pyrex spinbar conjugated with biorecognition elements to address these limitations. Advantages include an inherent capacity for effective mixing, an almost instantaneous recovery of the spinbar that can be performed without expensive equipment and with no loss of magnetic particles during processing, and reduced transfer of sample matrix. As a result, spinbars can provide an effective means for IMS with large-volume assays composed of complex matrices.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.)
Databáze: MEDLINE
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