Micrometer-thick and porous nanocomposite coating for electrochemical sensors with exceptional antifouling and electroconducting properties.

Autor: Lee JC; Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA, 02215, USA.; Department of Materials Science and Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, 34141, Republic of Korea., Kim SY; Department of Materials Science and Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, 34141, Republic of Korea., Song J; Bionanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, 34141, Republic of Korea.; Center for Systems Biology, Massachusetts General Hospital Research Institute, Boston, MA, 02114, USA.; Department of Radiology, Harvard Medical School, Boston, MA, 02114, USA., Jang H; Bionanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, 34141, Republic of Korea., Kim M; Department of Materials Science and Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, 34141, Republic of Korea., Kim H; Department of Chemical and Biomolecular Engineering, KAIST, Daejeon, 34141, Republic of Korea., Choi SQ; Department of Chemical and Biomolecular Engineering, KAIST, Daejeon, 34141, Republic of Korea., Kim S; Department of Laboratory Medicine, Gyeongsang National University Hospital, Gyeongsang National University College of Medicine, Jinju-si, Gyeongsangnam-do, 52727, Republic of Korea., Jolly P; Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA, 02215, USA., Kang T; Bionanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, 34141, Republic of Korea. kangtaejoon@kribb.re.kr.; School of Pharmacy, Sungkyunkwan University (SKKU), Suwon-si, Gyeongi-do, 16419, Republic of Korea. kangtaejoon@kribb.re.kr., Park S; Department of Materials Science and Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, 34141, Republic of Korea. stevepark@kaist.ac.kr., Ingber DE; Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA, 02215, USA. don.ingber@wyss.harvard.edu.; Vascular Biology Program and Department of Surgery, Boston Children's Hospital and Harvard Medical School, Boston, MA, 02115, USA. don.ingber@wyss.harvard.edu.; Harvard John A. Paulson School of Engineering and Applied Sciences, Harvard University, Cambridge, MA, 02138, USA. don.ingber@wyss.harvard.edu.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2024 Feb 08; Vol. 15 (1), pp. 711. Date of Electronic Publication: 2024 Feb 08.
DOI: 10.1038/s41467-024-44822-1
Abstrakt: Development of coating technologies for electrochemical sensors that consistently exhibit antifouling activities in diverse and complex biological environments over extended time is vital for effective medical devices and diagnostics. Here, we describe a micrometer-thick, porous nanocomposite coating with both antifouling and electroconducting properties that enhances the sensitivity of electrochemical sensors. Nozzle printing of oil-in-water emulsion is used to create a 1 micrometer thick coating composed of cross-linked albumin with interconnected pores and gold nanowires. The layer resists biofouling and maintains rapid electron transfer kinetics for over one month when exposed directly to complex biological fluids, including serum and nasopharyngeal secretions. Compared to a thinner (nanometer thick) antifouling coating made with drop casting or a spin coating of the same thickness, the thick porous nanocomposite sensor exhibits sensitivities that are enhanced by 3.75- to 17-fold when three different target biomolecules are tested. As a result, emulsion-coated, multiplexed electrochemical sensors can carry out simultaneous detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nucleic acid, antigen, and host antibody in clinical specimens with high sensitivity and specificity. This thick porous emulsion coating technology holds promise in addressing hurdles currently restricting the application of electrochemical sensors for point-of-care diagnostics, implantable devices, and other healthcare monitoring systems.
(© 2024. The Author(s).)
Databáze: MEDLINE
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