Effects of antimicrobial photodynamic therapy with photodithazine® on methicillin-resistant Staphylococcus aureus (MRSA): Studies in biofilms and experimental model with Galleria mellonella.

Autor: Souza BMN; Photobiology Applied to Health (PhotoBioS Lab), Universidade do Vale do Paraíba (UNIVAP), São José dos Campos, São Paulo, Brazil., Miñán AG; Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, La Plata 1900, Argentina. Electronic address: agminan@inifta.unlp.edu.ar., Brambilla IR; Photobiology Applied to Health (PhotoBioS Lab), Universidade do Vale do Paraíba (UNIVAP), São José dos Campos, São Paulo, Brazil., Pinto JG; Photobiology Applied to Health (PhotoBioS Lab), Universidade do Vale do Paraíba (UNIVAP), São José dos Campos, São Paulo, Brazil. Electronic address: juguerra@univap.br., Garcia MT; Department of Biosciences and Oral Diagnosis, Universidade Estadual Paulista (Unesp), Institute of Science and Technology (ICT), São José dos Campos, São Paulo, Brazil., Junqueira JC; Department of Biosciences and Oral Diagnosis, Universidade Estadual Paulista (Unesp), Institute of Science and Technology (ICT), São José dos Campos, São Paulo, Brazil. Electronic address: juliana.junqueira@unesp.br., Ferreira-Strixino J; Photobiology Applied to Health (PhotoBioS Lab), Universidade do Vale do Paraíba (UNIVAP), São José dos Campos, São Paulo, Brazil. Electronic address: juferreira@univap.br.
Jazyk: angličtina
Zdroj: Journal of photochemistry and photobiology. B, Biology [J Photochem Photobiol B] 2024 Mar; Vol. 252, pp. 112860. Date of Electronic Publication: 2024 Feb 03.
DOI: 10.1016/j.jphotobiol.2024.112860
Abstrakt: Staphylococcus aureus infections are a severe health problem due to the high mortality rate. Conventional treatment of these infections is via the administration of antibiotics. However, its indiscriminate use can select resistant microorganisms. Thus, it is necessary to develop alternatives for antibiotic therapy. Antimicrobial Photodynamic Therapy (aPDT), a therapeutic method that associates a photosensitizer (PS), a light source with adequate wavelength to the PS, interacts with molecular oxygen generating reactive oxygen species responsible for cell inactivation, is a viable alternative. This work aimed to analyze, in vitro and in vivo, the action of aPDT with PS Photodithazine® (PDZ) on the methicillin-resistant S. aureus (MRSA) strain. In the in vitro method, the S. aureus biofilm was incubated with PDZ at 50 and 75 μg.mL -1 for 15 min, adopting the light dose of 25, 50, and 100 J/cm 2 . In addition, PS interaction, formation of reactive oxygen species (ROS), bacterial metabolism, adhesion, bacterial viability, and biofilm structure were evaluated by scanning electron microscopy. Subsequently, the strain was inoculated into models of Galleria mellonella, and the survival curve, health scale, blood cell analysis, and CFU/mL of S. aureus in the hemolymph were analyzed after aPDT. In the in vitro results, bacterial reduction was observed in the different PDZ concentrations, highlighting the parameters of 75 μg.mL -1 of PDZ and 100 J/cm 2 . As for in vivo results, aPDT increased survival and stimulated the immune system of G. mellonella infected by S. aureus. aPDT proved effective in both models, demonstrating its potential as an alternative therapy in treating MRSA bacterial infections.
Competing Interests: Declaration of competing interest The authors whose names are listed immediately below certify that they have NO affiliations with or involvement in any organization or entity with any financial interest (such as honoraria; educational grants; participation in speakers' bureaus; membership, employment, consultancies, stock ownership, or other equity interest; and expert testimony or patent-licensing arrangements), or non-financial interest (such as personal or professional relationships, affiliations, knowledge or beliefs) in the subject matter or materials discussed in this manuscript.
(Copyright © 2023. Published by Elsevier B.V.)
Databáze: MEDLINE
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