Sustained antibacterial activity of orthodontic elastomeric ligature ties coated with a novel kombucha-derived bacterial nanocellulose: An in-vitro study.

Autor: Eskandari F; School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran., Borzou S; UCLA School of Dentistry, Los Angeles, CA, United States of America., Razavian A; Department of Endodontics, Semnan Dental School, Semnan University of Medical Sciences, Semnan, Iran., Babanouri N; Orthodontic Research Center, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Fars, Iran., Yousefi K; Department of Dental Materials and Biomaterials Research center, Shiraz Dental School, Shiraz University of Medical Sciences, Shiraz, Iran.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2024 Feb 08; Vol. 19 (2), pp. e0292966. Date of Electronic Publication: 2024 Feb 08 (Print Publication: 2024).
DOI: 10.1371/journal.pone.0292966
Abstrakt: Incipient carious lesions, the most common complication in orthodontic patients with fixed appliances, call for the development of novel preventive dental materials that do not rely on patient adherence. The present study aimed to assess the ability of elastomeric ligatures coated with bacterial nanocellulose (BNC) to deliver sustained antibacterial activity, during the standard 28-day interval between orthodontic appointments, without compromising their mechanical properties. Kombucha membrane was used to produce cellulose as a secondary product from the fermentation of tea broth with symbiotic bacteria and yeast culture. Characterization of BNC-coated elastomeric ligatures was performed using Fourier Transform Infrared Spectroscopy and Energy Dispersive Spectroscopy analysis. The samples were pre-treated by immersion first in isopropyl alcohol, then in 8 mL nanocellulose solution for 7 days. Tensile strain and strength of the BNC-coated and conventional ligatures were evaluated using a tensile testing machine. Direct contact and agar diffusion tests were performed to assess the antibacterial activity of nanocellulose. In addition, the release profile of BNC was evaluated. Data analysis was performed by one-way analysis of variance (ANOVA) followed by post-hoc Tukey's test and Wilcoxon signed-rank test. P values less than 0.05 was regarded as significant. There was no statistically significant difference in tensile strain and strength between the BNC-coated and conventional ligatures. The coated ligatures provided sustained antibacterial activity during the required 28 days. The use of BNC-coated elastomeric ligatures in patients with fixed orthodontic appliances might be a promising solution to plaque formation and subsequent enamel decalcification.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2024 Eskandari et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Databáze: MEDLINE
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