Granular Hydrogels Improve Myogenic Invasion and Repair after Volumetric Muscle Loss.

Autor: Tanner GI; Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN, 47907, USA.; Department of Agricultural and Biological Engineering, Purdue University, West Lafayette, IN, 47907, USA., Schiltz L; Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN, 47907, USA., Narra N; Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN, 47907, USA., Figueiredo ML; Department of Basic Medical Sciences, Purdue University, West Lafayette, IN, 47907, USA., Qazi TH; Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN, 47907, USA.
Jazyk: angličtina
Zdroj: Advanced healthcare materials [Adv Healthc Mater] 2024 Feb 08, pp. e2303576. Date of Electronic Publication: 2024 Feb 08.
DOI: 10.1002/adhm.202303576
Abstrakt: Skeletal muscle injuries including volumetric muscle loss (VML) lead to excessive tissue scarring and permanent functional disability. Despite its high prevalence, there is currently no effective treatment for VML. Bioengineering interventions such as biomaterials that fill the VML defect to support cell and tissue growth are a promising therapeutic strategy. However, traditional biomaterials developed for this purpose lack the pore features needed to support cell infiltration. The present study investigates for the first time, the impact of granular hydrogels on muscle repair - hypothesizing that their flowability will permit conformable filling of the defect site and their inherent porosity will support the invasion of native myogenic cells, leading to effective muscle repair. Small and large microparticle fragments are prepared from photocurable hyaluronic acid polymer via extrusion fragmentation and facile size sorting. In assembled granular hydrogels, particle size and degree of packing significantly influence pore features, rheological behavior, and injectability. Using a mouse model of VML, it is demonstrated that, in contrast to bulk hydrogels, granular hydrogels support early-stage (satellite cell invasion) and late-stage (myofiber regeneration) muscle repair processes. Together, these results highlight the promising potential of injectable and porous granular hydrogels in supporting endogenous repair after severe muscle injury.
(© 2024 The Authors. Advanced Healthcare Materials published by Wiley‐VCH GmbH.)
Databáze: MEDLINE
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