Detection of SARS-CoV-2 spike protein D614G mutation using μTGGE.

Autor: Juma KM; Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Sakyo-Ku, Kyoto, 606-8502, Japan., Morimoto K; Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Sakyo-Ku, Kyoto, 606-8502, Japan., Sharma V; BioSeeds Corporation, JAIST Venture Business Laboratory, Ishikawa Create Lab, Ashahidai 2-13, Nomi City, Ishikawa, 923-1211, Japan.; Biyani BioSolutions Pvt. Ltd., R-4, Sector 3, Vidhyadharnagar, Jaipur, 302023, India., Sharma K; BioSeeds Corporation, JAIST Venture Business Laboratory, Ishikawa Create Lab, Ashahidai 2-13, Nomi City, Ishikawa, 923-1211, Japan., Biyani R; BioSeeds Corporation, JAIST Venture Business Laboratory, Ishikawa Create Lab, Ashahidai 2-13, Nomi City, Ishikawa, 923-1211, Japan., Biyani M; BioSeeds Corporation, JAIST Venture Business Laboratory, Ishikawa Create Lab, Ashahidai 2-13, Nomi City, Ishikawa, 923-1211, Japan. biyani@jaist.ac.jp.; Biyani BioSolutions Pvt. Ltd., R-4, Sector 3, Vidhyadharnagar, Jaipur, 302023, India. biyani@jaist.ac.jp.; Department of Bioscience and Biotechnology, Japan Advanced Institute of Science and Technology, 1-1 Asahidai, Nomi City, Ishikawa, 923-1292, Japan. biyani@jaist.ac.jp., Takita T; Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Sakyo-Ku, Kyoto, 606-8502, Japan., Yasukawa K; Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Sakyo-Ku, Kyoto, 606-8502, Japan. yasukawa.kiyoshi.7v@kyoto-u.ac.jp.
Jazyk: angličtina
Zdroj: Molecular biology reports [Mol Biol Rep] 2024 Feb 08; Vol. 51 (1), pp. 289. Date of Electronic Publication: 2024 Feb 08.
DOI: 10.1007/s11033-023-09065-1
Abstrakt: Background: The accurate and expeditious detection of SARS-CoV-2 mutations is critical for monitoring viral evolution, assessing its impact on transmission, virulence, and vaccine efficacy, and formulating public health interventions. In this study, a detection system utilizing micro temperature gradient gel electrophoresis (μTGGE) was developed for the identification of the D614 and G614 variants of the SARS-CoV-2 spike protein.
Methods: The in vitro synthesized D614 and G614 gene fragments of the SARS-CoV-2 spike protein were amplified via polymerase chain reaction and subjected to μTGGE analysis.
Results: The migration patterns exhibited by the D614 and G614 variants on the polyacrylamide gel were distinctly dissimilar and readily discernible by μTGGE. In particular, the mid-melting pattern of D614 was shorter than that of G614.
Conclusions: Our results demonstrate the capability of μTGGE for the rapid, precise, and cost-effective detection of SARS-CoV-2 spike protein D614 and G614 variants without the need for sequencing. Therefore, this approach holds considerable potential for use in point-of-care mutation assays for SARS-CoV-2 and other pathogens.
(© 2024. The Author(s), under exclusive licence to Springer Nature B.V.)
Databáze: MEDLINE
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