Frontal noradrenergic and cholinergic transients exhibit distinct spatiotemporal dynamics during competitive decision-making.

Autor:	Wang H; Interdepartmental Neuroscience Program, Yale University School of Medicine, New Haven, Connecticut, 06511, USA., Ortega HK; Interdepartmental Neuroscience Program, Yale University School of Medicine, New Haven, Connecticut, 06511, USA., Kelly EB; Department of Psychiatry, Yale University School of Medicine, New Haven, Connecticut, 06511, USA.; Meinig School of Biomedical Engineering, Cornell University, Ithaca, New York, 14853, USA., Indajang J; Meinig School of Biomedical Engineering, Cornell University, Ithaca, New York, 14853, USA., Feng J; State Key Laboratory of Membrane Biology, Peking University School of Life Sciences, Beijing, China., Li Y; State Key Laboratory of Membrane Biology, Peking University School of Life Sciences, Beijing, China.; PKU-IDG/McGovern Institute for Brain Research, Beijing, China.; Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing, China.; Chinese Institute for Brain Research, Beijing, China., Kwan AC; Department of Psychiatry, Yale University School of Medicine, New Haven, Connecticut, 06511, USA.; Meinig School of Biomedical Engineering, Cornell University, Ithaca, New York, 14853, USA.; Department of Psychiatry, Weill Cornell Medicine, New York, New York, 10065, USA.
Jazyk:	angličtina
Zdroj:	BioRxiv : the preprint server for biology [bioRxiv] 2024 Jan 24. Date of Electronic Publication: 2024 Jan 24.
DOI:	10.1101/2024.01.23.576893
Abstrakt:	Norepinephrine (NE) and acetylcholine (ACh) are neuromodulators that are crucial for learning and decision-making. In the cortex, NE and ACh are released at specific sites along neuromodulatory axons, which would constrain their spatiotemporal dynamics at the subcellular scale. However, how the fluctuating patterns of NE and ACh signaling may be linked to behavioral events is unknown. Here, leveraging genetically encoded NE and ACh indicators, we use two-photon microscopy to visualize neuromodulatory signals in the superficial layer of the mouse medial frontal cortex during decision-making. Head-fixed mice engage in a competitive game called matching pennies against a computer opponent. We show that both NE and ACh transients carry information about decision-related variables including choice, outcome, and reinforcer. However, the two neuromodulators differ in their spatiotemporal pattern of task-related activation. Spatially, NE signals are more segregated with choice and outcome encoded at distinct locations, whereas ACh signals can multiplex and reflect different behavioral correlates at the same site. Temporally, task-driven NE transients were more synchronized and peaked earlier than ACh transients. To test functional relevance, using optogenetics we found that evoked elevation of NE, but not ACh, in the medial frontal cortex increases the propensity of the animals to switch and explore alternate options. Taken together, the results reveal distinct spatiotemporal patterns of rapid ACh and NE transients at the subcellular scale during decision-making in mice, which may endow these neuromodulators with different ways to impact neural plasticity to mediate learning and adaptive behavior. Competing Interests: Conflict of Interest A.C.K. has been a scientific advisor or consultant for Empyrean Neuroscience, Freedom Biosciences, and Psylo. A.C.K. has received research support from Intra-Cellular Therapies. These duties had no influence on the content of this article.
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu