Development of the hyolaryngeal architecture in horseshoe bats: insights into the evolution of the pulse generation for laryngeal echolocation.

Autor: Nojiri T; Graduate School of Medicine, Juntendo University, 2-2-1 Hongo, Bunkyo-Ku, Tokyo, 113-8421, Japan. nojiri0805@gmail.com., Takechi M; Graduate School of Medicine, Juntendo University, 2-2-1 Hongo, Bunkyo-Ku, Tokyo, 113-8421, Japan.; Department of Molecular Craniofacial Embryology, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-Ku, Tokyo, 113-8549, Japan., Furutera T; Graduate School of Medicine, Juntendo University, 2-2-1 Hongo, Bunkyo-Ku, Tokyo, 113-8421, Japan.; Department of Molecular Craniofacial Embryology, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-Ku, Tokyo, 113-8549, Japan., Brualla NLM; Department of Infectious Diseases and Public Health, Jockey Club College of Veterinary Medicine and Life Sciences, City University of Hong Kong, Hong Kong SAR, China., Iseki S; Department of Molecular Craniofacial Embryology, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-Ku, Tokyo, 113-8549, Japan., Fukui D; The University of Tokyo Fuji Iyashinomori Woodland Study Center, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 341-2 Yamanaka, Yamanakako, Yamanashi, 401-05013, Japan., Tu VT; Institute of Ecology and Biological Resources, Vietnam Academy of Science and Technology, No. 18, Hoang Quoc Viet Road, Cau Giay District, Hanoi, Vietnam.; Graduate University of Science and Technology, Vietnam Academy of Science and Technology, No. 18, Hoang Quoc Viet Road, Cau Giay District, Hanoi, Vietnam., Meguro F; Research and Development Center for Precision Medicine, University of Tsukuba, 1-2 Kasuga, Tsukuba-Shi, Ibaraki, 305-8550, Japan., Koyabu D; Department of Molecular Craniofacial Embryology, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-Ku, Tokyo, 113-8549, Japan. dsk8evoluxion@gmail.com.; Department of Infectious Diseases and Public Health, Jockey Club College of Veterinary Medicine and Life Sciences, City University of Hong Kong, Hong Kong SAR, China. dsk8evoluxion@gmail.com.; Research and Development Center for Precision Medicine, University of Tsukuba, 1-2 Kasuga, Tsukuba-Shi, Ibaraki, 305-8550, Japan. dsk8evoluxion@gmail.com.
Jazyk: angličtina
Zdroj: EvoDevo [Evodevo] 2024 Feb 07; Vol. 15 (1), pp. 2. Date of Electronic Publication: 2024 Feb 07.
DOI: 10.1186/s13227-024-00221-7
Abstrakt: Background: The hyolaryngeal apparatus generates biosonar pulses in the laryngeally echolocating bats. The cartilage and muscles comprising the hyolarynx of laryngeally echolocating bats are morphologically modified compared to those of non-bat mammals, as represented by the hypertrophied intrinsic laryngeal muscle. Despite its crucial contribution to laryngeal echolocation, how the development of the hyolarynx in bats differs from that of other mammals is poorly documented. The genus Rhinolophus is one of the most sophisticated laryngeal echolocators, with the highest pulse frequency in bats. The present study provides the first detailed description of the three-dimensional anatomy and development of the skeleton, cartilage, muscle, and innervation patterns of the hyolaryngeal apparatus in two species of rhinolophid bats using micro-computed tomography images and serial tissue sections and compares them with those of laboratory mice. Furthermore, we measured the peak frequency of the echolocation pulse in active juvenile and adult individuals to correspond to echolocation pulses with hyolaryngeal morphology at each postnatal stage.
Results: We found that the sagittal crests of the cricoid cartilage separated the dorsal cricoarytenoid muscle in horseshoe bats, indicating that this unique morphology may be required to reinforce the repeated closure movement of the glottis during biosonar pulse emission. We also found that the cricothyroid muscle is ventrally hypertrophied throughout ontogeny, and that the cranial laryngeal nerve has a novel branch supplying the hypertrophied region of this muscle. Our bioacoustic analyses revealed that the peak frequency shows negative allometry against skull growth, and that the volumetric growth of all laryngeal cartilages is correlated with the pulse peak frequency.
Conclusions: The unique patterns of muscle and innervation revealed in this study appear to have been obtained concomitantly with the acquisition of tracheal chambers in rhinolophids and hipposiderids, improving sound intensity during laryngeal echolocation. In addition, significant protrusion of the sagittal crest of the cricoid cartilage and the separated dorsal cricoarytenoid muscle may contribute to the sophisticated biosonar in this laryngeally echolocating lineage. Furthermore, our bioacoustic data suggested that the mineralization of these cartilages underpins the ontogeny of echolocation pulse generation. The results of the present study provide crucial insights into how the anatomy and development of the hyolaryngeal apparatus shape the acoustic diversity in bats.
(© 2024. The Author(s).)
Databáze: MEDLINE
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