Ciprofloxacin- and levofloxacin-loaded nanoparticles efficiently suppressed fluoroquinolone resistance and biofilm formation in Acinetobacter baumannii.

Autor: Aboelenin AM; Department of Microbiology and Immunology, Faculty of Pharmacy, Mansoura University, PO Box 35516, Mansoura, Egypt., El-Mowafy M; Department of Microbiology and Immunology, Faculty of Pharmacy, Mansoura University, PO Box 35516, Mansoura, Egypt., Saleh NM; Department of Pharmaceutics, Faculty of Pharmacy, Mansoura University, PO Box 35516, Mansoura, Egypt., Shaaban MI; Department of Microbiology and Immunology, Faculty of Pharmacy, Mansoura University, PO Box 35516, Mansoura, Egypt. mona_ibrahim@mans.edu.eg., Barwa R; Department of Microbiology and Immunology, Faculty of Pharmacy, Mansoura University, PO Box 35516, Mansoura, Egypt. rasha@mans.edu.eg.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2024 Feb 07; Vol. 14 (1), pp. 3125. Date of Electronic Publication: 2024 Feb 07.
DOI: 10.1038/s41598-024-53441-1
Abstrakt: The spread of fluoroquinolone (FQ) resistance in Acinetobacter baumannii represents a critical health threat. This study aims to overcome FQ resistance in A. baumannii via the formulation of polymeric nanoFQs. Herein, 80 A. baumannii isolates were obtained from diverse clinical sources. All A. baumannii isolates showed high resistance to most of the investigated antimicrobials, including ciprofloxacin (CIP) and levofloxacin (LEV) (97.5%). FQ resistance-determining regions of the gyrA and parC genes were the most predominant resistant mechanism, harbored by 69 (86.3%) and 75 (93.8%) of the isolates, respectively. Additionally, plasmid-mediated quinolone resistance genes aac(6')-Ib and qnrS were detected in 61 (76.3%) and 2 (2.5%) of the 80 isolates, respectively. The CIP- and LEV-loaded poly ε-caprolactone (PCL) nanoparticles, F CIP and F LEV , respectively, showed a 1.5-6- and 6-12-fold decrease in the MIC, respectively, against the tested isolates. Interestingly, the time kill assay demonstrated that MICs of F CIP and F LEV completely killed A. baumannii isolates after 5-6 h of treatment. Furthermore, F CIP and F LEV were found to be efficient in overcoming the FQ resistance mediated by the efflux pumps in A. baumannii isolates as revealed by decreasing the MIC four-fold lower than that of free CIP and LEV, respectively. Moreover, F CIP and F LEV at 1/2 and 1/4 MIC significantly decreased biofilm formation by 47-93% and 69-91%, respectively. These findings suggest that polymeric nanoparticles can restore the effectiveness of FQs and represent a paradigm shift in the fight against A. baumannii isolates.
(© 2024. The Author(s).)
Databáze: MEDLINE
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