Altered luteal expression patterns of genomic and non-genomic progesterone receptors in bitches at different reproductive states.

Autor: Ucar EH; Aydin Adnan Menderes University, Faculty of Veterinary Medicine, Department of Obstetrics and Gynecology, Aydin, Turkey. Electronic address: hakan.ucar@adu.edu.tr., Peker C; Aydin Adnan Menderes University, Faculty of Veterinary Medicine, Department of Obstetrics and Gynecology, Aydin, Turkey. Electronic address: cevdet.peker@adu.edu.tr., Hitit M; Kastamonu University, Faculty of Veterinary Medicine, Department of Animal Genetics, Kastamonu, Turkey; Prairie View University, College of Agriculture, Food and Human Sciences, Prairie View, TX, USA. Electronic address: vetdrmustafahitit@gmail.com., Kose M; Dicle University, Faculty of Veterinary Medicine, Department of Obstetrics and Gynecology, Diyarbakir, Turkey. Electronic address: mehmetkose1977@gmail.com., Tatar M; Kastamonu University, Faculty of Veterinary Medicine, Department of Histology and Emrbyology, Kastamonu, Turkey. Electronic address: mtatar@kastamonu.edu.tr., Bozkaya F; Harran University, Faculty of Veterinary Medicine, Department of Animal Science and Animal Nutrition/Department of Veterinary Genetics, Sanliurfa, Turkey. Electronic address: farukbozkaya@yahoo.com., Atli MO; Harran University, Faculty of Veterinary Medicine, Department of Reproduction and Artificial Inseminatio, Sanliurfa, Turkey. Electronic address: moatli@hotmail.com.
Jazyk: angličtina
Zdroj: Theriogenology [Theriogenology] 2024 Apr 01; Vol. 218, pp. 153-162. Date of Electronic Publication: 2024 Feb 02.
DOI: 10.1016/j.theriogenology.2024.02.001
Abstrakt: The binding of steroid hormones to their specific receptors is necessary to exert their effects on target cells. Progesterone (P 4 ), a steroid hormone, carries out its effects through both genomic and non-genomic (the cell membrane-associated) receptors. This study aimed to ascertain luteal expression patterns of genomic and non-genomic progesterone receptors in bitches in physiological (early dioestrus and early pregnant) and pathological (pyometra) reproductive states. Luteal tissue was collected from the bitches at early dioestrus (ED, n = 5), early pregnant (EP, n = 5), and pyometra (PY, n = 5). The expression profiles of Steroidogenic Acute Regulator Protein (STAR), Progesterone Receptor (PGR), Membrane Progestin Receptors (PAQR5, PAQR7 and PAQR8), and Progesterone Membrane Components (PGMRC1 and PGMRC2) were examined at the mRNA levels using Real-Time Polymerase Chain Reaction (RT-PCR). Protein levels of PGR, PGMRC1 and PGMRC2 were detected by western blotting (WB). The STAR expression was found in all groups, with a statistical difference observed between EP and PY groups (P < 0.05). The protein level of PGR was determined to be highest in the EP group and lowest in the PY group. The expression of PAQR8 increased in the EP group (P < 0.05). The PAQR5 exhibited high expression in the EP group and low expression in the PY group (P < 0.05). PGRMC1 was more elevated in the EP group and lower in the PY group (P < 0.05). Protein levels of PGMRC1 and PGMRC2 were also observed at the highest expression in EP group. According to the altered expression profiles for examined receptors, we suggest that those progesterone receptors have roles in early pregnancy or pyometra in bitches.
Competing Interests: Declaration of competing interest The authors declare no conflict of interest.
(Copyright © 2024 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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