Analysis of nonsynonymous SNPs in candidate genes that influence bovine temperament and evaluation of their effect in Brahman cattle.

Autor: Ruiz-De-La-Cruz G; Laboratorio de Biotecnología Animal, Centro de Biotecnología Genómica, Instituto Politécnico Nacional, Reynosa, Tamaulipas, 88710, México., Sifuentes-Rincón AM; Laboratorio de Biotecnología Animal, Centro de Biotecnología Genómica, Instituto Politécnico Nacional, Reynosa, Tamaulipas, 88710, México. asifuentes@ipn.mx., Paredes-Sánchez FA; Unidad Académica Multidisciplinaria Mante, Universidad Autónoma de Tamaulipas, El Mante, Tamaulipas, 89840, México., Parra-Bracamonte GM; Laboratorio de Biotecnología Animal, Centro de Biotecnología Genómica, Instituto Politécnico Nacional, Reynosa, Tamaulipas, 88710, México., Casas E; National Animal Disease Center, Agricultural Research Service, United States Department of Agriculture, Ames, IA, 50010, USA., Riley DG; Department of Animal Science, Texas A&M University, College Station, TX, 77843, USA., Perry GA; Texas A&M AgriLife Research, Overton, TX, 75684, USA., Welsh TH Jr; Department of Animal Science, Texas A&M University, College Station, TX, 77843, USA., Randel RD; Texas A&M AgriLife Research, Overton, TX, 75684, USA.
Jazyk: angličtina
Zdroj: Molecular biology reports [Mol Biol Rep] 2024 Feb 07; Vol. 51 (1), pp. 285. Date of Electronic Publication: 2024 Feb 07.
DOI: 10.1007/s11033-024-09264-4
Abstrakt: Background: Temperament is an important production trait in cattle and multiple strategies had been developed to generate molecular markers to assist animal selection. As nonsynonymous single nucleotide polymorphisms are markers with the potential to affect gene functions, they could be useful to predict phenotypic effects. Genetic selection of less stress-responsive, temperamental animals is desirable from an economic and welfare point of view.
Methods and Results: Two nonsynonymous single nucleotide polymorphisms identified in HTR1B and SLC18A2 candidate genes for temperament were analyzed in silico to determine their effects on protein structure. Those nsSNPs allowing changes in proteins were selected for a temperament association analysis in a Brahman population. Transversion effects on protein structure were evaluated in silico for each amino acid change model, revealing structural changes in the proteins of the HTR1B and SLC18A2 genes. The selected nsSNPs were genotyped in a Brahman population (n = 138), and their genotypic effects on three temperament traits were analyzed: exit velocity, pen score, and temperament score. Only the SNP rs209984404-HTR1B (C/A) showed a significant association (P = 0.0144) with pen score. The heterozygous genotype showed a pen score value 1.17 points lower than that of the homozygous CC genotype.
Conclusion: The results showed that in silico analysis could direct the selection of nsSNPs with the potential to change the protein. Non-synonymous single nucleotide polymorphisms causing structural changes and reduced protein stability were identified. Only rs209984404-HTR1B shows that the allele affecting protein stability was associated with the genotype linked to docility in cattle.
(© 2024. The Author(s).)
Databáze: MEDLINE
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