The bHLH transcription factor OsPRI1 activates the Setaria viridis PEPC1 promoter in rice.

Autor: Carvalho P; Instituto de Tecnologia Química e Biológica António Xavier da Universidade Nova de Lisboa (ITQB NOVA), 2780-157, Oeiras, Portugal., Gomes C; Instituto de Tecnologia Química e Biológica António Xavier da Universidade Nova de Lisboa (ITQB NOVA), 2780-157, Oeiras, Portugal., Gonçalves I; Instituto de Tecnologia Química e Biológica António Xavier da Universidade Nova de Lisboa (ITQB NOVA), 2780-157, Oeiras, Portugal., Lourenço TF; Instituto de Tecnologia Química e Biológica António Xavier da Universidade Nova de Lisboa (ITQB NOVA), 2780-157, Oeiras, Portugal., Vlad D; Department of Biology, University of Oxford, South Parks Rd, OX1 3RB, Oxford, UK., Langdale JA; Department of Biology, University of Oxford, South Parks Rd, OX1 3RB, Oxford, UK., Saibo NJM; Instituto de Tecnologia Química e Biológica António Xavier da Universidade Nova de Lisboa (ITQB NOVA), 2780-157, Oeiras, Portugal.; Instituto de Biologia Experimental e Tecnológica (iBET), 2780-157, Oeiras, Portugal.
Jazyk: angličtina
Zdroj: The New phytologist [New Phytol] 2024 Mar; Vol. 241 (6), pp. 2495-2505. Date of Electronic Publication: 2024 Feb 07.
DOI: 10.1111/nph.19556
Abstrakt: Photosynthetic efficiency is reduced by the dual role of Rubisco, which acts either as a carboxylase or as an oxygenase, the latter leading to photorespiration. C 4 photosynthesis evolved as a carbon-concentrating mechanism to reduce photorespiration. To engineer C 4 into a C 3 plant, it is essential to understand how C 4 genes, such as phosphoenolpyruvate carboxylase (PEPC1), are regulated to be expressed at high levels and in a cell-specific manner. Yeast one-hybrid screening was used to show that OsPRI1, a rice bHLH transcription factor involved in iron homeostasis, binds to the Setaria viridis PEPC1 promoter. This promoter drives mesophyll-specific gene expression in rice. The role of OsPRI1 in planta was characterized using a rice line harbouring SvPEPC1 pro ::GUS. We show that OsPRI1 activates the S. viridis PEPC1 promoter by binding to an N-box in the proximal promoter, and that GUS activity is highly reduced in SvPEPC1 pro ::GUS lines when OsPRI1 is mutated. Cross-species comparisons showed that the SvPRI1 homolog binds to the SvPEPC1 promoter but the maize ZmPRI1 does not bind to the ZmPEPC1 promoter. Our results suggest that elements of the iron homeostasis pathway were co-opted to regulate PEPC1 gene expression during the evolution of some but not all C 4 species.
(© 2024 The Authors. New Phytologist © 2024 New Phytologist Foundation.)
Databáze: MEDLINE
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