| Autor: |
Taravat M; Department of Clinical Science, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran., Asadpour R; Department of Clinical Science, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran., Jafari Jozani R; Department of Clinical Science, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran., Fattahi A; Department of Reproductive Biology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran., Khordadmehr M; Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran., Hajipour H; Department of Clinical Science, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran. |
| Jazyk: |
angličtina |
| Zdroj: |
Systems biology in reproductive medicine [Syst Biol Reprod Med] 2024 Dec; Vol. 70 (1), pp. 3-19. Date of Electronic Publication: 2024 Feb 07. |
| DOI: |
10.1080/19396368.2024.2306420 |
| Abstrakt: |
Endometritis is an inflammatory and histopathologic disease in uterine tissues that interferes with the proper decidualization and implantation of the embryo. In this study, rosmarinic acid (RA) is used as an anti-inflammatory agent that encapsulates in exosomes and is used to attenuate lipopolysaccharide (LPS)-induced endometritis and improve implantation. For this purpose, exosomes were loaded with RA and then administrated into the animal groups, including RA, exosome, RA plus exosome (RA + Exo), and RA-loaded exosomes (RALExo) groups. The concentrations of RA or exosomes used in this study were 10 mg/kg, and the compounds were injected into the uterine horn 24 h following the induction of endometritis. Upon the presence of inflammation detected by the histopathological method, the most proper groups were mated with male mice. The effect of the treatment group on the implantation rate, progesterone levels, and gene expressions were assessed by Chicago Blue staining, enzyme-linked immunosorbent assay (ELISA), and Quantitative PCR (qPCR), respectively. Results showed RALExo10 and RA10 + Exo10 groups improved pathological alterations, enhanced progesterone levels, increased implantation rate, as well as heightened expression levels of Leukemia inhibitory factor (LIF) and Mucin-16 ( MUC-16 ) genes. Besides, the expression levels of inflammatory cytokines, including Transforming growth factor-β (TGF-β), Interlukine-10 (IL-10), Interlukine-15 (IL-15), and Interlukine-18 (IL-18), were regulated. Our findings indicated that the expression of LIF, Muc-16 genes as well as IL - 18, were significantly correlated with serum progesterone concentrations and the implantation rate in the treatment groups. The RALExo10 and RA10 + Exo10 groups showed ameliorated implantation rates in experimental groups. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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