Plasma-Derived Fibrin Hydrogels Containing Graphene Oxide for Infections Treatment.

Autor:	Martín C; Department of Bioengineering, Universidad Carlos III de Madrid, Leganés 28911, Spain., Bachiller A; Department of Bioengineering, Universidad Carlos III de Madrid, Leganés 28911, Spain., Fernández-Blázquez JP; Institute IMDEA Materials, Getafe 28906, Spain., Nishina Y; Graduate School of Natural Science and Technology, Okayama University, Okayama 700-8530, Japan.; Research Core for Interdisciplinary Sciences, Okayama University, Okayama 700-8530, Japan., Jorcano JL; Department of Bioengineering, Universidad Carlos III de Madrid, Leganés 28911, Spain.
Jazyk:	angličtina
Zdroj:	ACS materials letters [ACS Mater Lett] 2023 Mar 23; Vol. 5 (4), pp. 1245-1255. Date of Electronic Publication: 2023 Mar 23 (Print Publication: 2023).
DOI:	10.1021/acsmaterialslett.2c01044
Abstrakt:	Wound infection is inevitable in most patients suffering from extensive burns or chronic ulcers, and there is an urgent demand for the production of bactericidal dressings to be used as grafts to restore skin functionalities. In this context, the present study explores the fabrication of plasma-derived fibrin hydrogels containing bactericidal hybrids based on graphene oxide (GO). The hydrogels were fully characterized regarding gelation kinetics, mechanical properties, and internal hydrogel structures by disruptive cryo scanning electron microscopies (cryo-SEMs). The gelation kinetic experiments revealed an acceleration of the gel formation when GO was added to the hydrogels in a concentration of up to 0.2 mg/mL. The cryo-SEM studies showed up a decrease of the pore size when GO was added to the network, which agreed with a faster area contraction and a higher compression modulus of the hydrogels that contained GO, pointing out the critical structural role of the nanomaterial. Afterward, to study the bactericidal ability of the gels, GO was used as a carrier, loading streptomycin (STREP) on its surface. The loading content of the drug to form the hybrid (GO/STREP) resulted in 50.2% ± 4.7%, and the presence of the antibiotic was also demonstrated by Raman spectroscopy, Z-potential studies, and thermogravimetric analyses. The fibrin-derived hydrogels containing GO/STREP showed a dose-response behavior according to the bactericidal hybrid concentration and allowed a sustained release of the antibiotic at a programmed rate, leading to drug delivery over a prolonged period of time. Competing Interests: The authors declare no competing financial interest. (© 2023 American Chemical Society.)
Databáze:	MEDLINE
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