Proteomic profiles of Leptospira borgpetersenii serovar Hardjo strains JB197 and HB203 cultured at different temperatures.

Autor: Putz EJ; Infectious Bacterial Disease Research Unit, USDA Agriculture Research Service, National Animal Disease Center, Ames, IA, USA. Electronic address: ellie.putz@usda.gov., Fernandes LGV; Infectious Bacterial Disease Research Unit, USDA Agriculture Research Service, National Animal Disease Center, Ames, IA, USA., Sarlo Davila KM; Infectious Bacterial Disease Research Unit, USDA Agriculture Research Service, National Animal Disease Center, Ames, IA, USA., Whitelegge J; The Pasarow Mass Spectrometry Laboratory, David Geffen School of Medicine, NPI-Semel Institute for Neuroscience and Human Behavior, University of California, Los Angeles, Los Angeles, CA, United States., Lippolis JD; Ruminant Diseases and Immunology Research Unit, USDA Agriculture Research Service, National Animal Disease Center, Ames, IA, USA., Nally JE; Infectious Bacterial Disease Research Unit, USDA Agriculture Research Service, National Animal Disease Center, Ames, IA, USA.
Jazyk: angličtina
Zdroj: Journal of proteomics [J Proteomics] 2024 Mar 20; Vol. 295, pp. 105106. Date of Electronic Publication: 2024 Feb 05.
DOI: 10.1016/j.jprot.2024.105106
Abstrakt: Leptospirosis is a global zoonotic disease affecting humans, domestic, and wild animals. Leptospira are typically shed in the urine of reservoir hosts which persist in suitable environments where incidental host transmission occurs after direct contact with infected urine or contaminated environments. Interestingly, serologically identical L. borgpetersenii serovar Hardjo strains JB197 and HB203 show divergent disease severity in the hamster model; JB197 causes severe acute infection while HB203 causes persistent chronic infection. Historically, serovar Hardjo was limited to culture at 29 °C, but utilization of HAN media allows propagation from host tissues at 37 °C. Here, the proteome of strains JB197 and HB203 were characterized after culture from experimentally challenged hamsters at 29 °C and 37 °C. Comparative analyses of JB197 and HB203 samples cultured at 29 °C yielded 425 significantly differentially expressed (DE) proteins, while strains at 37 °C yielded 613 DE proteins including prominent outer membrane proteins and known virulence factors. In agreement, membrane protein GO terms were identified by STRING network analyses along with numerous metabolic KEGG pathways consistent with condition differences. Within strain, JB197 cultured at 29 °C vs 37 °C identified 529 DE proteins, while HB203 identified 524 DE proteins. Investigating differential protein profiles provide insights into strain specific behaviors with implications for better understanding host-pathogen interactions, disease transmission, and response to environmental conditions which can contribute to vaccine development, diagnostic improvement, and ultimately leptospirosis control. SIGNIFICANCE: Leptospirosis is a devastating zoonotic disease affecting humans, wild and domestic animals around the globe. Different species and serovars of Leptospira can affect various animal host species differently; for instance, a serovar that is asymptomatic in the rat may cause severe disease in a dog or human. These differences in host response are not only found at the species and serovar level for Leptospira, but also at the strain level. A prime example comes from strains JB197 and HB203, both species L. borgpetersenii, both serovar Hardjo. Interestingly, JB197 causes a severe acute infection in the hamster while HB203 causes an asymptomatic chronic infection. Understanding these unique relationships between pathogen and host species is important, especially in the context of prevention technologies such as vaccine design, where the strain of Leptospira used as a bacterin might have different efficiencies in different hosts. In this study, proteomic profiles of strains JB197 and HB203 were analyzed, and results revealed diverse protein expression profiles of outer membrane proteins, as well as proteins functioning in motility and growth.
Competing Interests: Declaration of competing interest The authors have no competing interests to report.
(Published by Elsevier B.V.)
Databáze: MEDLINE
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