Co-occurrence network analysis reveals the alterations of the skin microbiome and metabolome in adults with mild to moderate atopic dermatitis.

Autor:	P Gomes PW; Collaborative Mass Spectrometry Innovation Center, Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California San Diego, La Jolla, California, USA., Mannochio-Russo H; Collaborative Mass Spectrometry Innovation Center, Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California San Diego, La Jolla, California, USA., Mao J; Colgate-Palmolive Company, Piscataway, New Jersey, USA., Zhao HN; Collaborative Mass Spectrometry Innovation Center, Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California San Diego, La Jolla, California, USA., Ancira J; RTL Genomics, MicroGenDX, Lubbock, Texas, USA., Tipton CD; RTL Genomics, MicroGenDX, Lubbock, Texas, USA., Dorrestein PC; Collaborative Mass Spectrometry Innovation Center, Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California San Diego, La Jolla, California, USA.; Department of Pediatrics, University of California, San Diego, California, USA., Li M; Colgate-Palmolive Company, Piscataway, New Jersey, USA.
Jazyk:	angličtina
Zdroj:	MSystems [mSystems] 2024 Mar 19; Vol. 9 (3), pp. e0111923. Date of Electronic Publication: 2024 Feb 06.
DOI:	10.1128/msystems.01119-23
Abstrakt:	Skin microbiome can be altered in patients with atopic dermatitis (AD). An understanding of the changes from healthy to atopic skin can help develop new targets for treatment by identifying microbial and molecular biomarkers. This study investigates the skin microbiome and metabolome of healthy adult subjects and lesion (ADL) and non-lesion (ADNL) of AD patients by 16S rRNA gene sequencing and mass spectrometry, respectively. Samples from AD patients showed alterations in the diversity and composition of the skin microbiome, with ADL skin having the greatest divergence. Staphylococcus species, especially S. aureus , were significantly increased in AD patients. Metabolomic profiles were also different between the groups. Dipeptide derivatives are more abundant in ADL, which may be related to skin inflammation. Co-occurrence network analysis of the microbiome and metabolomics data revealed higher co-occurrence of metabolites and bacteria in healthy ADNL compared to ADL. S. aureus co-occurred with dipeptide derivatives in ADL, while phytosphingosine-derived compounds showed co-occurrences with commensal bacteria, for example , Paracoccus sp., Pseudomonas sp., Prevotella bivia , Lactobacillus iners , Anaerococcus sp., Micrococcus sp., Corynebacterium ureicelerivorans , Corynebacterium massiliense , Streptococcus thermophilus , and Roseomonas mucosa , in healthy and ADNL groups. Therefore, these findings provide valuable insights into how AD affects the human skin metabolome and microbiome.IMPORTANCEThis study provides valuable insight into changes in the skin microbiome and associated metabolomic profiles in an adult population with mild to moderate atopic dermatitis. It also identifies new therapeutic targets that may be useful for developing personalized treatments for individuals with atopic dermatitis based on their unique skin microbiome and metabolic profiles. Competing Interests: P.C.D. is a scientific advisor to Cybele and a co-founder and advisor and holds equity in Ometa, Arome, and Enveda with prior approval by the UC-San Diego. M.L. and J.M. work for Colgate-Palmolive, which financially supported the study. C.D.T. and J.A. are employees of RTL Genomics and were compensated by Colgate-Palmolive for their contribution to the 16S sequencing analysis.
Databáze:	MEDLINE
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