Ultrastructural and morphological studies on variables affecting Escherichia coli with selected commercial antibiotics.

Autor: Muhamad Hendri NA; Electron Microscopy Unit, Special Resource Centre (SRC), Institute for Medical Research, Ministry of Health Malaysia, Setia Alam, 40170 Selangor, Malaysia., Nor Amdan NA; Bacteriology Unit, Infectious Disease Research Centre (IDRC), Institute for Medical Research, Ministry of Health Malaysia, Setia Alam, 40170 Selangor, Malaysia., Dounis SO; Electron Microscopy Unit, Special Resource Centre (SRC), Institute for Medical Research, Ministry of Health Malaysia, Setia Alam, 40170 Selangor, Malaysia., Sulaiman Najib N; Electron Microscopy Unit, Special Resource Centre (SRC), Institute for Medical Research, Ministry of Health Malaysia, Setia Alam, 40170 Selangor, Malaysia., Louis SR; Electron Microscopy Unit, Special Resource Centre (SRC), Institute for Medical Research, Ministry of Health Malaysia, Setia Alam, 40170 Selangor, Malaysia.
Jazyk: angličtina
Zdroj: Cell surface (Amsterdam, Netherlands) [Cell Surf] 2024 Jan 14; Vol. 11, pp. 100120. Date of Electronic Publication: 2024 Jan 14 (Print Publication: 2024).
DOI: 10.1016/j.tcsw.2024.100120
Abstrakt: Background: Many studies reported the effects of antibiotic exposure on E. coli bacterial growth and cell modification. However, scarce descriptive information on ultrastructural effects upon exposure of commercial antibiotics.
Methods: This study described the morphological and ultrastructural alterations caused by selected antibiotics (amoxicillin-clavulanate, ceftriaxone, polymyxin B, colistin, gentamicin, and amikacin) that targeted cell wall, plasma membrane, and cytoplasmic density, and also proteins synthesis. We determined extracellular morphological changes of exposure through scanning electron microscopy (FESEM) and intracellular activities through transmission electron microscopy (TEM) investigation.
Results: FESEM and TEM micrograph of E. coli exposed with selected antibiotics shows ultrastructural changes in beta-lactam class (amoxicillin-clavulanate, ceftriaxone) elongated the cells as the cell wall was altered as it inhibits bacterial cell wall synthesis, polymyxin class (polymyxin B, colistin) had plasmid and curli-fimbriae as it breaking down the plasma/cytoplasmic membrane, and aminoglycoside class (gentamicin, and amikacin) reduced ribosome concentration as it inhibits bacterial protein synthesis by binding to 30 s ribosomes.
Conclusion: Morphological and ultrastructural alterations of E. coli 's mechanism of actions were translated and depicted. This study could be reference for characterization studies for morphological and ultrastructural of E. coli upon exposure to antimicrobial agents.
Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(© 2024 The Authors.)
Databáze: MEDLINE
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