Non-structural protein 1 and hematology parameters as predictors of dengue virus infection severity in Indonesia.

Autor: Sunari IGAAEP; Department of Clinical Pathology, Faculty of Medicine, Dr. Soetomo Teaching Hospital, Universitas Airlangga, Surabaya, Indonesia., Aryati A; Department of Clinical Pathology, Faculty of Medicine, Dr. Soetomo Teaching Hospital, Universitas Airlangga, Surabaya, Indonesia., Hakim FKN; Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia., Tanzilia MF; Department of Clinical Pathology, Faculty of Medicine, Dr. Soetomo Teaching Hospital, Universitas Airlangga, Surabaya, Indonesia., Zuroidah N; Department of Clinical Pathology, Faculty of Medicine, Dr. Soetomo Teaching Hospital, Universitas Airlangga, Surabaya, Indonesia., Wrahatnala BJ; Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia., Rohman A; Department of Chemistry, Faculty of Science and Technology, Universitas Airlangga, Surabaya, Indonesia., Wardhani P; Department of Clinical Pathology, Faculty of Medicine, Dr. Soetomo Teaching Hospital, Universitas Airlangga, Surabaya, Indonesia., Husada D; Department of Child Health, Faculty of Medicine, Dr. Soetomo Teaching Hospital, Universitas Airlangga, Surabaya, Indonesia., Miftahussurur M; Department of Internal Medicine, Division of Gastroentero-Hepatology, Faculty of Medicine, Dr. Soetomo Teaching Hospital, Universitas Airlangga, Surabaya, Indonesia.; Helicobacter pylori and Microbiota Study Group Institute of Tropical Disease, Universitas Airlangga, Surabaya, Indonesia.
Jazyk: angličtina
Zdroj: Journal of medicine and life [J Med Life] 2023 Oct; Vol. 16 (10), pp. 1546-1551.
DOI: 10.25122/jml-2022-0300
Abstrakt: Dengue virus infection (DVI) remains a significant health challenge, and diagnosis must still be considered. Non-structural protein 1 (NS1) is a potential marker of the dengue virus that can help diagnose DVI. The study aimed to assess the role of NS1 as a predictor of the severity of DVI. We utilized Dengue PCR-confirmed samples and employed semi-quantitative NS1Ag ELISA for NS1 examination, adhering to the World Health Organization South-East Asia Region (WHO-SEARO) 2011 criteria for DVI. We included DVI patients from Indonesia aged 1-65 years. Secondary infections had more severe clinical conditions than primary infections. Leukocyte and platelet levels had a more significant effect on NS1 positivity (6.19 (1.9-30.2); p<0.001; 190 (11-417); p=0.015; respectively). Multivariate analysis revealed leukocytes as a more significant predictor of NS1 values than platelets, with an odds ratio of 5.38 contributing to 30.5% of the NS1 value variation. The NS1 value could distinguish undifferentiated fever and dengue fever in the children group with a sensitivity of 76.0% and specificity of 87.5% (p=0.015). The number of NS1(-) in the severe dengue hemorrhagic fever (DHF) group was higher than NS1(+). DENV-4 type and primary infection were dominant in this study, although they did not significantly differ from the NS1 value. NS1 value can be used as a predictor to determine the severity of DVI in children but not in the adult group. The levels of leukocytes and platelets influenced the NS1 value.
Competing Interests: The authors declare no conflict of interest.
(© 2023 The Author(s).)
Databáze: MEDLINE
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