Tunable PhenoCycler imaging of the murine pre-clinical tumour microenvironments.

Autor: Abraham MJ; Lady Davis Institute, Jewish General Hospital, Montreal, QC, Canada.; Division of Experimental Medicine, McGill University, Montreal, QC, Canada., Goncalves C; Lady Davis Institute, Jewish General Hospital, Montreal, QC, Canada., McCallum P; Lady Davis Institute, Jewish General Hospital, Montreal, QC, Canada.; Division of Experimental Medicine, McGill University, Montreal, QC, Canada., Gupta V; Lady Davis Institute, Jewish General Hospital, Montreal, QC, Canada.; Division of Experimental Medicine, McGill University, Montreal, QC, Canada.; University of British Columbia, Vancouver, BC, Canada., Preston SEJ; Lady Davis Institute, Jewish General Hospital, Montreal, QC, Canada.; Division of Experimental Medicine, McGill University, Montreal, QC, Canada., Huang F; Lady Davis Institute, Jewish General Hospital, Montreal, QC, Canada.; Division of Experimental Medicine, McGill University, Montreal, QC, Canada., Chou H; Lady Davis Institute, Jewish General Hospital, Montreal, QC, Canada.; Clinical Research Unit, Jewish General Hospital, Montreal, QC, Canada., Gagnon N; Lady Davis Institute, Jewish General Hospital, Montreal, QC, Canada., Johnson NA; Lady Davis Institute, Jewish General Hospital, Montreal, QC, Canada.; Division of Experimental Medicine, McGill University, Montreal, QC, Canada.; Clinical Research Unit, Jewish General Hospital, Montreal, QC, Canada., Miller WH; Lady Davis Institute, Jewish General Hospital, Montreal, QC, Canada. wilson.miller@mcgill.ca.; Division of Experimental Medicine, McGill University, Montreal, QC, Canada. wilson.miller@mcgill.ca.; Clinical Research Unit, Jewish General Hospital, Montreal, QC, Canada. wilson.miller@mcgill.ca., Mann KK; Lady Davis Institute, Jewish General Hospital, Montreal, QC, Canada. koren.mann@mcgill.ca.; Division of Experimental Medicine, McGill University, Montreal, QC, Canada. koren.mann@mcgill.ca.; Department of Pharmacology and Therapeutics, McGill University, Montreal, QC, Canada. koren.mann@mcgill.ca., Del Rincon SV; Lady Davis Institute, Jewish General Hospital, Montreal, QC, Canada. soniavictoria.delrincon@mcgill.ca.; Division of Experimental Medicine, McGill University, Montreal, QC, Canada. soniavictoria.delrincon@mcgill.ca.
Jazyk: angličtina
Zdroj: Cell & bioscience [Cell Biosci] 2024 Feb 04; Vol. 14 (1), pp. 19. Date of Electronic Publication: 2024 Feb 04.
DOI: 10.1186/s13578-024-01199-4
Abstrakt: Background: The tumour microenvironment (TME) consists of tumour-supportive immune cells, endothelial cells, and fibroblasts. PhenoCycler, a high-plex single cell spatial biology imaging platform, is used to characterize the complexity of the TME. Researchers worldwide harvest and bank tissues from mouse models which are employed to model a plethora of human disease. With the explosion of interest in spatial biology, these panoplies of archival tissues provide a valuable resource to answer new questions. Here, we describe our protocols for developing tunable PhenoCycler multiplexed imaging panels and describe our open-source data analysis pipeline. Using these protocols, we used PhenoCycler to spatially resolve the TME of 8 routinely employed pre-clinical models of lymphoma, breast cancer, and melanoma preserved as FFPE.
Results: Our data reveal distinct TMEs in the different cancer models that were imaged and show that cell-cell contacts differ depending on the tumour type examined. For instance, we found that the immune infiltration in a murine model of melanoma is altered in cellular organization in melanomas that become resistant to αPD-1 therapy, with depletions in a number of cell-cell interactions.
Conclusions: This work presents a valuable resource study seamlessly adaptable to any field of research involving murine models. The methodology described allows researchers to address newly formed hypotheses using archival materials, bypassing the new to perform new mouse studies.
(© 2024. The Author(s).)
Databáze: MEDLINE
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