Inheritance of genomic regions and genes associated with number of oocytes and embryos in Gir cattle through daughter design.

Autor:	Rocha RFB; Department of Animal Science, Universidade Federal de Viçosa, Viçosa, MG, 36570-900, Brazil; Animal Breeding and Genomics, Wageningen University & Research, 6700 AH Wageningen, the Netherlands., Garcia AO; Department of Animal Science, Universidade Federal de Viçosa, Viçosa, MG, 36570-900, Brazil., Dos Santos MG; Department of Animal Science, Universidade Federal de Viçosa, Viçosa, MG, 36570-900, Brazil., Otto PI; Department of Animal Science, Universidade Federal de Santa Maria, Santa Maria, RS, 97105-900, Brazil., da Silva MVB; EMBRAPA-Dairy Cattle, Juiz de Fora, MG, 36038-330, Brazil., Martins MF; EMBRAPA-Dairy Cattle, Juiz de Fora, MG, 36038-330, Brazil., Machado MA; EMBRAPA-Dairy Cattle, Juiz de Fora, MG, 36038-330, Brazil., Panetto JCC; EMBRAPA-Dairy Cattle, Juiz de Fora, MG, 36038-330, Brazil., Calus MPL; Animal Breeding and Genomics, Wageningen University & Research, 6700 AH Wageningen, the Netherlands., Guimarães SEF; Department of Animal Science, Universidade Federal de Viçosa, Viçosa, MG, 36570-900, Brazil. Electronic address: sfacioni@ufv.br.
Jazyk:	angličtina
Zdroj:	Journal of dairy science [J Dairy Sci] 2024 Jun; Vol. 107 (6), pp. 3794-3801. Date of Electronic Publication: 2024 Feb 02.
DOI:	10.3168/jds.2023-24111
Abstrakt:	Over the past decades, daughter designs, including genotyped sires and their genotyped daughters, have been used as an approach to identify QTL related to economic traits. The aim of this study was to identify genomic regions inherited by Gir sire families and genes associated with number of viable oocytes (VO), total number of oocytes (TO), and number of embryos (EMBR) based on a daughter design approach. In total, 15 Gir sire families were selected. The number of daughters per family ranged from 26 to 395, which were genotyped with different SNP panels and imputed to the Illumina BovineHD BeadChip (777K) and had phenotypes for oocyte and embryo production. Daughters had phenotypic data for VO, TO, and EMBR. The search for QTL was performed through GWAS based on GBLUP. The QTL were found for each trait among and within families based on the top 10 genomic windows with the greatest genetic variance. For EMBR, genomic windows identified among families were located on BTA4, BTA5, BTA6, BTA7, BTA8, BTA13, BTA16, and BTA17, and they were most frequent on BTA7 within families. For VO, genomic windows were located on BTA2, BTA4, BTA5, BTA7, BTA17, BTA21, BTA22, BTA23, and BTA27 among families, being most frequent on BTA8 within families. For TO, the top 10 genomic windows were identified on BTA2, BTA4, BTA5, BTA7, BTA17, BTA21, BTA22, BTA26, and BTA27, being most frequent on BTA7 and BTA8 within families. Considering all results, the greatest number of genomic windows was found on BTA7, where the VCAN, XRCC4, TRNAC-ACA, HAPLN1, and EDIL3 genes were identified in the common regions. In conclusion, 15 Gir sire families with 26 to 395 daughters per family with phenotypes for oocyte and embryo production helped to identify the inheritance of several genomic regions, especially on BTA7, where the EDIL3, HAPLN1, and VCAN candidate genes were associated with number of oocytes and embryos in Gir cattle families. (The Authors. Published by Elsevier Inc. on behalf of the American Dairy Science Association®. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).)
Databáze:	MEDLINE
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