The construction and immunogenicity analyses of a recombinant pseudorabies virus with Senecavirus A VP3 protein co-expression.

Autor: Tao Q; College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China., Xu L; College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China., Zhang Y; College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China., Yang Y; College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China., Liu Z; College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China., Xu T; College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China., Lai S; College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China., Ai Y; College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China., Zhu L; College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, China. Electronic address: abtczl72@126.com., Xu Z; College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, China. Electronic address: abtcxzw@126.com.
Jazyk: angličtina
Zdroj: Veterinary microbiology [Vet Microbiol] 2024 Mar; Vol. 290, pp. 110011. Date of Electronic Publication: 2024 Jan 24.
DOI: 10.1016/j.vetmic.2024.110011
Abstrakt: Senecavirus A (SVA)-associated porcine idiopathic vesicular disease (PIVD) and Pseudorabies (PR) are highly contagious swine disease that pose a significant threat to the global pig industry. In the absence of an effective commercial vaccine, outbreaks caused by SVA have occurred in many parts of the world. In this study, the PRV variant strain PRV-XJ was used as the parental strain to construct a recombinant PRV strain with the TK/gE/gI proteins deletion and the VP3 protein co-expression, named rPRV-XJ-ΔTK/gE/gI-VP3. The results revealed that PRV is a suitable viral live vector for VP3 protein expressing. As a vaccine, rPRV-XJ-ΔTK/gE/gI-VP3 is safe for mice, vaccination with it did not cause any clinical symptoms of PRV. Intranasal immunization with rPRV-XJ-ΔTK/gE/gI-VP3 induced strong cellular immune response and high levels of specific antibody against VP3 and gB and neutralizing antibodies against both PRV and SVA in mice. It provided 100% protection to mice against the challenge of virulent strain PRV-XJ, and alleviated the pathological lesion of heart and liver tissue in SVA infected mice. rPRV-XJ-ΔTK/gE/gI-VP3 appears to be a promising vaccine candidate against PRV and SVA for the control of the PRV variant and SVA.
Competing Interests: Declaration of Competing Interest The authors declare that they have no conflict of interest.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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