Changes in splenic tissue and immune response profile of Schistosoma mansoni infected mice submitted to chronic ethanol intake.
Autor: | Rosa AAD; Romero Lascasas Porto Laboratory of Helminthology, Department of Microbiology, Immunology and Parasitology, Faculty of Medical Sciences, Rio de Janeiro State University, Brazil., Brandão-Bezerra L; Romero Lascasas Porto Laboratory of Helminthology, Department of Microbiology, Immunology and Parasitology, Faculty of Medical Sciences, Rio de Janeiro State University, Brazil., Corrêa CL; Department of Pathology and Laboratories, School of Medical Sciences, Rio de Janeiro State University, Brazil; Medicine School, Estácio de Sá University, Brazil., Amaral G Da-Silva S; Laboratory of Parasitic Immunopharmacology, Department of Microbiology, Immunology and Parasitology, Faculty of Medical Sciences, Rio de Janeiro State University, Brazil., Rodrigues LS; Laboratory of Immunopathology, Department of Pathology and Laboratories, Faculty of Medical Sciences, Rio de Janeiro State University, Brazil., Machado-Silva JR; Romero Lascasas Porto Laboratory of Helminthology, Department of Microbiology, Immunology and Parasitology, Faculty of Medical Sciences, Rio de Janeiro State University, Brazil., Neves RH; Romero Lascasas Porto Laboratory of Helminthology, Department of Microbiology, Immunology and Parasitology, Faculty of Medical Sciences, Rio de Janeiro State University, Brazil. Electronic address: renataheisler@gmail.com. |
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Jazyk: | angličtina |
Zdroj: | Experimental parasitology [Exp Parasitol] 2024 Apr; Vol. 259, pp. 108706. Date of Electronic Publication: 2024 Feb 01. |
DOI: | 10.1016/j.exppara.2024.108706 |
Abstrakt: | In Schistosoma mansoni infection, the spleen is one of the organs affected, causing its enlargement (splenomegaly). Intake of ethanol through alcoholic beverages can cause spleen atrophy and interfere with immune activity. To gain knowledge of this association on the spleen and on the immune response profile, male mice were used as an experimental model. These animals were divided into four groups: C. control; EC. uninfected/ethanol gavage; I. infected; and IE. infected/ethanol gavage. Groups I and IE were infected with about 100 cercariae (BH strain) of S. mansoni and in the fifth week of infection, gavage 200 μL/day/animal of 18 % ethanol was started for 28 consecutive days. At the end of the gavage (9th week of infection) all animals were euthanized. The spleen was removed and longitudinally divided in two parts. After histological processing, the sections were stained with H&E and Gomori's Reticulin for histopathological and stereological analyses, white pulp morphometry and quantification of megakaryocytes. The other fragment was macerated (in laminar flow) and the cell suspension, after adjusting the concentration (2 × 10 6 ), was plated to obtain cytokines produced by spleen cells that were measured by flow cytometry (Citometric Bead Array). Histopathological and quantitative analyzes in the spleen of the IE group showed an increase in the number of trabeculae and megakaryocytes, a decrease in reticular fibers, as well as important organizational changes in the white pulp and red pulp. Due to the decrease in the levels of cytokines measured and the result of the calculation of the ratio between the IFN-y and IL-10 cytokines (p = 0.0079) of the infected groups, we suggest that ethanol decreased the inflammatory and anti-inflammatory response generated by the infection (group IE, the production of cytokines was significantly decreased (p < 0.01). These changes demonstrate that ethanol ingestion interferes with some parameters of experimental S. mansoni infection, such as changes in splenic tissue and in the pattern of cytokine production. Competing Interests: Declaration of competing interest We have no conflict of interest to declare. (Copyright © 2024 Elsevier Inc. All rights reserved.) |
Databáze: | MEDLINE |
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