Molecular epidemiology and antimicrobial resistance of vaginal Candida glabrata isolates in Namibia.

Autor:	Dunaiski CM; Namibia University of Sciences and Technology, Department of Health and Applied Sciences, Windhoek 10005, Namibia.; University of Pretoria, Department of Medical Microbiology, Pretoria 0001, South Africa., Kock MM; University of Pretoria, Department of Medical Microbiology, Pretoria 0001, South Africa.; National Health Laboratory Service, Tshwane, Academic Division, Pretoria 3191, South Africa., Chan WY; Sequencing Core Facility, National Institute for Communicable Diseases a Division of the National Health Laboratory Service, Johannesburg 2131, South Africa.; Department of Biochemistry, Genetics and Microbiology, University of Pretoria, Pretoria 0081, South Africa.; Right to care, Centurion 0157, South Africa., Ismail A; Sequencing Core Facility, National Institute for Communicable Diseases a Division of the National Health Laboratory Service, Johannesburg 2131, South Africa.; Department of Biochemistry and Microbiology, Faculty of Science, Engineering and Agriculture, University of Venda, Thohoyandou 0950, South Africa.; Institute for Water and Wastewater Technology, Durban University of Technology, Durban 4000, South Africa., Peters RPH; University of Pretoria, Department of Medical Microbiology, Pretoria 0001, South Africa.; University of Cape Town, Division of Medical Microbiology, Cape Town 7701, South Africa.; Foundation for Professional Development, Research Unit, East London 5217, South Africa.
Jazyk:	angličtina
Zdroj:	Medical mycology [Med Mycol] 2024 Jan 27; Vol. 62 (2).
DOI:	10.1093/mmy/myae009
Abstrakt:	Candida glabrata is the most common non-albicans Candida species that causes vulvovaginal candidiasis (VVC). Given the intrinsically low susceptibility of C. glabrata to azole drugs, investigations into C. glabrata prevalence, fungal susceptibility profile, and molecular epidemiology are necessary to optimise the treatment of VVC. This molecular epidemiological study was conducted to determine antifungal drug profile, single nucleotide polymorphisms (SNPs) associated with phenotypic antifungal resistance and epidemic diversity of C. glabrata isolates from women with VVC in Namibia. Candida glabrata isolates were identified using phenotypic and molecular methods. Antifungal susceptibility of strains was determined for fluconazole, itraconazole, amphotericin B, and anidulafungin. Whole genome sequencing was used to determine SNPs in antifungal resistance genes and sequence type (ST) allocation. Among C. glabrata isolates, all (20/20; 100%) exhibited phenotypic resistance to the azole class antifungal drug, (fluconazole), and phenotypic susceptibility to the polyene class (amphotericin B), and the echinocandins (anidulafungin). Non-synonymous SNPs were identified in antifungal resistance genes of all fluconazole-resistant C. glabrata isolates including ERG6 (15%), ERG7 (15%), CgCDR1 (25%), CgPDR1 (60%), SNQ2 (10%), FKS1 (5.0%), FKS2 (5.0%), CgFPS1 (5.0%), and MSH2 (15%). ST15 (n = 8/20, 40%) was predominant. This study provides important insight into phenotypic and genotypic antifungal resistance across C. glabrata isolates from women with VVC in Namibia. In this study, azole resistance is determined by an extensive range of SNPs, while the observed polyene and echinocandin resistance-associated SNPs despite phenotypic susceptibility require further investigation. (© The Author(s) 2024. Published by Oxford University Press on behalf of The International Society for Human and Animal Mycology.)
Databáze:	MEDLINE
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