Exploring advances in single particle CryoEM with apoferritin: From blobs to true atomic resolution.

Autor: Premageetha GT; Institute for Stem Cell Science and Regenerative Medicine, GKVK Post, Bangalore 560065, India; Manipal Academy of Higher Education, Tiger Circle Road, Manipal, Karnataka 576104, India. Electronic address: tpgowtham@instem.res.in., Vinothkumar KR; National Centre for Biological Sciences, Tata Institute of Fundamental Research, GKVK Post, Bangalore 560065, India., Bose S; Institute for Stem Cell Science and Regenerative Medicine, GKVK Post, Bangalore 560065, India. Electronic address: sucharitab@instem.res.in.
Jazyk: angličtina
Zdroj: The international journal of biochemistry & cell biology [Int J Biochem Cell Biol] 2024 Apr; Vol. 169, pp. 106536. Date of Electronic Publication: 2024 Feb 01.
DOI: 10.1016/j.biocel.2024.106536
Abstrakt: Deciphering the three-dimensional structures of macromolecules is of paramount importance for gaining insights into their functions and roles in human health and disease. Single particle cryoEM has emerged as a powerful technique that enables direct visualization of macromolecules and their complexes, and through subsequent averaging, achieve near atomic-level resolution. A major breakthrough was recently achieved with the determination of the apoferritin structure at true atomic resolution. In this review, we discuss the latest technological innovations across the entire single-particle workflow, which have been instrumental in driving the resolution revolution and in transforming cryoEM as a mainstream technique in structural biology. We illustrate these advancements using apoferritin as an example that has served as an excellent benchmark sample for assessing emerging technologies. We further explore whether the existing technology can routinely generate atomic structures of dynamic macromolecules that more accurately represent real-world samples, the limitations in the workflow, and the current approaches employed to overcome them.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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