Diversity of Staphylococcus aureus associated with mastitis from dairy cows in Rwanda.

Autor: Keinprecht H; Institute of Microbiology, University of Veterinary Medicine Vienna, Vienna, Austria; New Vision Veterinary Hospital (NVVH) Northern Province, Musanze District, Rwanda., Irimaso E; School of Veterinary Medicine- CAVM, University of Rwanda, Nyagatare Campus, Nyagatare, Rwanda, Rwanda., Rosel AC; Austrian Agency for Health and Food Safety (AGES), Institute of Medical Microbiology and Hygiene, Vienna, Austria., Stessl B; Unit of Food Microbiology, Institute of Food Safety, Food Technology and Veterinary Public Health, University of Veterinary Medicine Vienna, Vienna, Austria., Ntakirutimana C; New Vision Veterinary Hospital (NVVH) Northern Province, Musanze District, Rwanda., Marek L; Institute of Microbiology, University of Veterinary Medicine Vienna, Vienna, Austria; New Vision Veterinary Hospital (NVVH) Northern Province, Musanze District, Rwanda., Fischer OW; New Vision Veterinary Hospital (NVVH) Northern Province, Musanze District, Rwanda., Szostak MP; Institute of Microbiology, University of Veterinary Medicine Vienna, Vienna, Austria., Zöchbauer J; Institute of Microbiology, University of Veterinary Medicine Vienna, Vienna, Austria., Wittek T; Clinical Unit of Ruminant Medicine, University of Veterinary Medicine, Vienna, Austria., Müller E; Leibniz Institute of Photonic Technology (IPHT), Jena, Germany; InfectoGnostics Research Campus, Jena, Germany., Desvars-Larrive A; Unit of Veterinary Public Health and Epidemiology, Institute of Food Safety, Food Technology and Veterinary Public Health, University of Veterinary Medicine Vienna, Vienna, Austria; Complexity Science Hub Vienna, Vienna, Austria., Feßler AT; Institute of Microbiology and Epizootics, Centre for Infection Medicine, School of Veterinary Medicine, Freie Universität Berlin, Berlin, Germany; Veterinary Centre for Resistance Research (TZR), School of Veterinary Medicine, Freie Universität Berlin, Berlin, Germany., Braun SD; Leibniz Institute of Photonic Technology (IPHT), Jena, Germany; InfectoGnostics Research Campus, Jena, Germany., Schwarz S; Institute of Microbiology and Epizootics, Centre for Infection Medicine, School of Veterinary Medicine, Freie Universität Berlin, Berlin, Germany; Veterinary Centre for Resistance Research (TZR), School of Veterinary Medicine, Freie Universität Berlin, Berlin, Germany., Spergser J; Institute of Microbiology, University of Veterinary Medicine Vienna, Vienna, Austria., Ehling-Schulz M; Institute of Microbiology, University of Veterinary Medicine Vienna, Vienna, Austria., Monecke S; Leibniz Institute of Photonic Technology (IPHT), Jena, Germany; InfectoGnostics Research Campus, Jena, Germany; Institut für Medizinische Mikrobiologie und Hygiene, Universitätsklinik Dresden, Dresden, Germany., Ehricht R; Leibniz Institute of Photonic Technology (IPHT), Jena, Germany; InfectoGnostics Research Campus, Jena, Germany; Institute of Physical Chemistry, Friedrich Schiller University Jena, Jena, Germany., Ruppitsch W; Austrian Agency for Health and Food Safety (AGES), Institute of Medical Microbiology and Hygiene, Vienna, Austria., Grunert T; Institute of Microbiology, University of Veterinary Medicine Vienna, Vienna, Austria., Loncaric I; Institute of Microbiology, University of Veterinary Medicine Vienna, Vienna, Austria. Electronic address: igor.loncaric@vetmeduni.ac.at.
Jazyk: angličtina
Zdroj: Journal of global antimicrobial resistance [J Glob Antimicrob Resist] 2024 Mar; Vol. 36, pp. 326-335. Date of Electronic Publication: 2024 Feb 01.
DOI: 10.1016/j.jgar.2024.01.017
Abstrakt: Objectives: The objective of the present study was to examine the diversity of Staphylococcus aureus from mastitis milk samples of cows in Rwanda.
Methods: A total of 1080 quarter milk samples from 279 dairy cows were collected in 80 different farms from all five provinces of Rwanda. In total, 135 S. aureus isolates were obtained and subjected to genotyping (spa typing, DNA microarray, whole-genome sequencing (WGS)), antimicrobial susceptibility testing (AST) and phenotypic profiling by Fourier Transform Infrared (FTIR) spectroscopy (including capsular serotyping).
Results: Resistance to penicillin and/or tetracycline was most frequently observed. Ten sequence types (STs) (ST1, ST151, ST152, ST5477, ST700, ST7110, ST7983, ST7984, ST8320, ST97) belonging to seven clonal complexes (CCs) (CC1, CC130, CC152, CC3591, CC3666, CC705, CC97) were detected. The Panton-Valentine leukocidin (PVL) genes (lukF-PV/lukS-PV), the bovine leukocidin genes (lukM/lukF-P83) and the human and bovine toxic shock syndrome toxin gene tst-1 variants were detected. FTIR-based capsular serotyping showed CC-specific differences. Most CC97 (cap5 allele) isolates were primarily nonencapsulated (82%), whereas isolates of CC3591 and CC3666 (cap8 allele) were mostly encapsulated (86.4% and 57.8%, respectively). Our results underline the widespread global distribution of cattle-adapted CC97.
Conclusion: The presence of CC3591 and CC3666 in bovine mastitis suggests an important role in cattle health and dairy production in Rwanda. The results of the present study support the need for a rigorous One-Health Surveillance program of the bovine-human interface.
(Copyright © 2024 The Author(s). Published by Elsevier Ltd.. All rights reserved.)
Databáze: MEDLINE
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