Large scale transcriptional analysis of MHC class I haplotype diversity in sheep.

Autor: Vasoya D; Division of Infection and Immunity, The Roslin Institute, The University of Edinburgh, Scotland, UK., Connelley T; Division of Infection and Immunity, The Roslin Institute, The University of Edinburgh, Scotland, UK., Tzelos T; Division of Infection and Immunity, The Roslin Institute, The University of Edinburgh, Scotland, UK.; Moredun Research Institute, Pentlands Science Park, Scotland, UK., Todd H; Moredun Research Institute, Pentlands Science Park, Scotland, UK., Ballingall KT; Moredun Research Institute, Pentlands Science Park, Scotland, UK.
Jazyk: angličtina
Zdroj: HLA [HLA] 2024 Feb; Vol. 103 (2), pp. e15356.
DOI: 10.1111/tan.15356
Abstrakt: Domestic sheep (Ovis aries) have been an important component of livestock agricultural production for thousands of years. Preserving genetic diversity within livestock populations maintains a capacity to respond to changing environments and rapidly evolving pathogens. MHC genetic diversity can influence immune functionality at individual and population levels. Here, we focus on defining functional MHC class I haplotype diversity in a large cohort of Scottish Blackface sheep pre-selected for high levels of MHC class II DRB1 diversity. Using high-throughput amplicon sequencing with three independent sets of barcoded primers we identified 134 MHC class I transcripts within 38 haplotypes. Haplotypes were identified with between two and six MHC class I genes, plus variable numbers of conserved sequences with very low read frequencies. One or two highly transcribed transcripts dominate each haplotype indicative of two highly polymorphic, classical MHC class I genes. Additional clusters of medium, low, and very low expressed transcripts are described, indicative of lower transcribed classical, non-classical and genes whose function remains to be determined.
(© 2024 The Authors. HLA: Immune Response Genetics published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
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