Pulsed electrical stimulation and amino acid derivatives promote collagen gene expression in human dermal fibroblasts.

Autor: Urabe H; Homer Ion Laboratory Co., Ltd, 17-2 Shinsen-cho, Shibuya-ku, Tokyo, 150-0045 Japan., Akimoto R; Homer Ion Laboratory Co., Ltd, 17-2 Shinsen-cho, Shibuya-ku, Tokyo, 150-0045 Japan., Kamiya S; Homer Ion Laboratory Co., Ltd, 17-2 Shinsen-cho, Shibuya-ku, Tokyo, 150-0045 Japan., Hosoki K; Homer Ion Laboratory Co., Ltd, 17-2 Shinsen-cho, Shibuya-ku, Tokyo, 150-0045 Japan., Ichikawa H; Homer Ion Laboratory Co., Ltd, 17-2 Shinsen-cho, Shibuya-ku, Tokyo, 150-0045 Japan., Nishiyama T; Homer Ion Laboratory Co., Ltd, 17-2 Shinsen-cho, Shibuya-ku, Tokyo, 150-0045 Japan.; Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183-8509 Japan.
Jazyk: angličtina
Zdroj: Cytotechnology [Cytotechnology] 2024 Feb; Vol. 76 (1), pp. 139-151. Date of Electronic Publication: 2023 Nov 09.
DOI: 10.1007/s10616-023-00604-z
Abstrakt: Several collagen types are important for maintaining skin structure and function. Previous reports show that l-hydroxyproline (Hyp), N-acetyl-l-hydroxyproline (AHyp), and l-alanyl-l-glutamine (Aln-Gln) are biological active substances with collagen synthesis-promoting effects. In this study, we combined the promotive effects of pulsed electrical stimulation (PES) with three amino acid derivatives in human dermal fibroblasts. Fibroblasts were exposed to PES with a 4,800 Hz pulse frequency and a voltage at 1 or 5 V for 15 min. The gene expression of type I and III collagen (fibrillar collagen), type IV and VII collagen (basement membrane collagen and anchoring fibril collagen) were measured by RT-PCR 48 h after PES. PES alone promoted the expression of COL1A1 and COL3A1 at 5 V but did not alter that of COL4A1 and COL7A1 . Each AAD and the AAD mixture promoted the expression of COL4A1 and COL7A1 but either repressed, or did not alter, that of COL1A1 and COL3A1 . Compared to treatment with each AAD, PES at 5 V with Hyp promoted the expression of COL1A1 and COL3A1 , enhanced COL3A1 expression with AHyp, and stimulated COL3A1 expression with Aln-Gln, while COL4A1 and COL7A1 expressions were not affected. PES and the AAD mixture significantly promoted COL4A1 expression in a voltage-dependent manner, and COL1A1 and COL3A1 demonstrated a similar but nonsignificant trend, whereas COL7A1 expression was not affected. The combination of PES with each AAD or the AAD mixture may improve skin structure and function by increasing the expression of basement membrane collagen and dermal fibrillar collagen.
Competing Interests: Competing interestsThe authors declare no competing interests.
(© The Author(s), under exclusive licence to Springer Nature B.V. 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.)
Databáze: MEDLINE
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