Combining microfluidic chip and low-attachment culture devices to isolate oral cancer stem cells.

Autor:	Chen HH; School of Dentistry, China Medical University, Taichung, Taiwan., Nguyen TV; School of Dentistry, China Medical University, Taichung, Taiwan., Shih YH; Department of Healthcare Administration, Asia University, Taichung, Taiwan., Chang KC; School of Dentistry, China Medical University, Taichung, Taiwan., Chiu KC; Division of General Dentistry, Taichung Armed Forces General Hospital, Taichung, Taiwan., Hsia SM; School of Nutrition and Health Sciences, Taipei Medical University, Taipei, Taiwan.; Nutrition Research Center, Taipei Medical University Hospital, Taipei, Taiwan., Fuh LJ; School of Dentistry, China Medical University, Taichung, Taiwan.; Department of Dentistry, China Medical University Hospital, Taichung, Taiwan., Shieh TM; School of Dentistry, China Medical University, Taichung, Taiwan.
Jazyk:	angličtina
Zdroj:	Journal of dental sciences [J Dent Sci] 2024 Jan; Vol. 19 (1), pp. 560-567. Date of Electronic Publication: 2023 Oct 14.
DOI:	10.1016/j.jds.2023.10.005
Abstrakt:	Background/purpose: Cancer stem cells (CSCs) are widely recognized as key drivers of cancer initiation, progression, and therapeutic resistance. Microfluidic chip technology offers a promising approach for CSC isolation and study. This study investigated the efficacy of a microfluidic chip-based method for isolating single cells from oral cancer cell lines characterized by high stem-like phenotypes. Specifically, the study focused on examining the sphere-forming capability and the expression of CSC markers, including aldehyde dehydrogenase 1A1 (ALDH1A1), CD44, and CD133, in isolated cell clones from OECM-1 and SAS cell lines. Materials and Methods: Oral cancer cell lines were subjected to isolation using a microfluidic chip. The captured single cells were cultured to assess their sphere-forming capacity in ultra-low binding culture. Furthermore, the protein expression levels of ALDH1A1, CD44, and CD133 in the isolated cell clones were analyzed using western blotting. Results: The microfluidic chip-assisted isolation method significantly enhanced the sphere-forming capability of both OECM-1 and SAS cell clones compared to their parent cell lines. Moreover, the expression levels of CSC markers ALDH1A1, CD44, and CD133 were upregulated in the microfluidic chip-assisted isolated cell clones, indicating a higher stem-like phenotype. Conclusion: This study demonstrates the effectiveness of the microfluidic chip-based approach in isolating oral cancer cell clones with elevated stem-like characteristics. This method offers a valuable tool for further investigation of CSCs and their role in cancer progression, as well as future therapy development for oral cancers. Competing Interests: The authors have no conflicts of interest relevant to this article. (© 2023 Association for Dental Sciences of the Republic of China. Publishing services by Elsevier B.V.)
Databáze:	MEDLINE
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