Adrenalectomy attenuates hyperalgesia but does not regulate muscle wasting in a female rat model of fibromyalgia.

Autor: Costa DM; Laboratory of Basic and Behavioral Neuroendocrinology (LANBAC), Department of Physiology, Center for Biological and Health Sciences, Federal University of Sergipe, São Cristóvão, Brazil.; Graduate Program in Physiological Sciences, Federal University of Sergipe, São Cristóvão, Brazil., da Silva RP; Laboratory of Basic and Behavioral Neuroendocrinology (LANBAC), Department of Physiology, Center for Biological and Health Sciences, Federal University of Sergipe, São Cristóvão, Brazil.; Graduate Program in Physiological Sciences, Federal University of Sergipe, São Cristóvão, Brazil., da Cruz-Filho J; Laboratory of Basic and Behavioral Neuroendocrinology (LANBAC), Department of Physiology, Center for Biological and Health Sciences, Federal University of Sergipe, São Cristóvão, Brazil.; Graduate Program in Physiological Sciences, Federal University of Sergipe, São Cristóvão, Brazil., de Oliveira Santos T; Laboratory of Basic and Behavioral Neuroendocrinology (LANBAC), Department of Physiology, Center for Biological and Health Sciences, Federal University of Sergipe, São Cristóvão, Brazil.; Graduate Program in Physiological Sciences, Federal University of Sergipe, São Cristóvão, Brazil., Dos Anjos-Santos HC; Laboratory of Basic and Behavioral Neuroendocrinology (LANBAC), Department of Physiology, Center for Biological and Health Sciences, Federal University of Sergipe, São Cristóvão, Brazil.; Graduate Program in Physiological Sciences, Federal University of Sergipe, São Cristóvão, Brazil., de Lucca W Jr; Department of Morphology, Center for Biological and Health Sciences, Federal University of Sergipe, São Cristóvão, Brazil., do Carmo Kettelhut Í; Department of Physiology and Biochemistry and Immunology, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, Brazil., Navegantes LC; Department of Physiology and Biochemistry and Immunology, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, Brazil., de Souza PRM; Graduate Program in Physiological Sciences, Federal University of Sergipe, São Cristóvão, Brazil., Camargo EA; Graduate Program in Physiological Sciences, Federal University of Sergipe, São Cristóvão, Brazil., Lauton-Santos S; Graduate Program in Physiological Sciences, Federal University of Sergipe, São Cristóvão, Brazil., Badauê-Passos D Jr; Laboratory of Basic and Behavioral Neuroendocrinology (LANBAC), Department of Physiology, Center for Biological and Health Sciences, Federal University of Sergipe, São Cristóvão, Brazil., Mecawi AS; Department of Biophysics, São Paulo Medical School, Federal University of São Paulo, São Paulo, Brazil., DeSantana JM; Graduate Program in Physiological Sciences, Federal University of Sergipe, São Cristóvão, Brazil., Lustrino D; Laboratory of Basic and Behavioral Neuroendocrinology (LANBAC), Department of Physiology, Center for Biological and Health Sciences, Federal University of Sergipe, São Cristóvão, Brazil.; Graduate Program in Physiological Sciences, Federal University of Sergipe, São Cristóvão, Brazil.
Jazyk: angličtina
Zdroj: Clinical and experimental pharmacology & physiology [Clin Exp Pharmacol Physiol] 2024 Mar; Vol. 51 (3), pp. e13837.
DOI: 10.1111/1440-1681.13837
Abstrakt: Although it is well established that fibromyalgia (FM) syndrome is characterized by chronic diffuse musculoskeletal hyperalgesia, very little is known about the effect of this pathology on muscle tissue plasticity. Therefore, the present study aimed to characterize the putative alterations in skeletal muscle mass in female rats subjected to a FM model by inducing chronic diffuse hyperalgesia (CDH) through double injections of acidic saline (pH 4.0) into the left gastrocnemius muscle at 5-day intervals. To determine protein turnover, the total proteolysis, proteolytic system activities and protein synthesis were evaluated in oxidative soleus muscles of pH 7.2 (control) and pH 4.0 groups at 7 days after CDH induction. All animals underwent behavioural analyses of mechanical hyperalgesia, strength and motor performance. Our results demonstrated that, in addition to hyperalgesia, rats injected with acidic saline exhibited skeletal muscle loss, as evidenced by a decrease in the soleus fibre cross-sectional area. This muscle loss was associated with increased proteasomal proteolysis and expression of the atrophy-related gene (muscle RING-finger protein-1), as well as reduced protein synthesis and decreased protein kinase B/S6 pathway activity. Although the plasma corticosterone concentration did not differ between the control and pH 4.0 groups, the removal of the adrenal glands attenuated hyperalgesia, but it did not prevent the increase in muscle protein loss in acidic saline-injected animals. The data suggests that the stress-related hypothalamic-pituitary-adrenal axis is involved in the development of hyperalgesia, but is not responsible for muscle atrophy observed in the FM model induced by intramuscular administration of acidic saline. Although the mechanisms involved in the attenuation of hyperalgesia in rats injected with acidic saline and subjected to adrenalectomy still need to be elucidated, the results found in this study suggest that glucocorticoids may not represent an effective therapeutic approach to alleviate FM symptoms.
(© 2024 John Wiley & Sons Australia, Ltd.)
Databáze: MEDLINE
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