Anti-migratory and cytotoxic effect of indole derivative in C6 glioma cells.

Autor: Andrade AKS; Physiological Sciences Graduate Program, Federal University of Sergipe, São Cristóvão, Sergipe, Brazil; Morphology Department, Federal University of Sergipe, São Cristóvão, Sergipe, Brazil., de Franca MNF; Health Science Graduate Program, Federal University of Sergipe, Aracaju, Sergipe, Brazil; Morphology Department, Federal University of Sergipe, São Cristóvão, Sergipe, Brazil. Electronic address: mariananobre@academico.ufs.br., Santos JF; Physiological Sciences Graduate Program, Federal University of Sergipe, São Cristóvão, Sergipe, Brazil; Morphology Department, Federal University of Sergipe, São Cristóvão, Sergipe, Brazil., Macêdo NA; Pharmacy Department, Federal University of Sergipe, São Cristóvão, Sergipe, Brazil., de Lucca Junior W; Morphology Department, Federal University of Sergipe, São Cristóvão, Sergipe, Brazil., Scher R; Morphology Department, Federal University of Sergipe, São Cristóvão, Sergipe, Brazil., Cavalcanti SCH; Pharmacy Department, Federal University of Sergipe, São Cristóvão, Sergipe, Brazil., Corrêa CB; Physiological Sciences Graduate Program, Federal University of Sergipe, São Cristóvão, Sergipe, Brazil; Health Science Graduate Program, Federal University of Sergipe, Aracaju, Sergipe, Brazil; Morphology Department, Federal University of Sergipe, São Cristóvão, Sergipe, Brazil.
Jazyk: angličtina
Zdroj: Toxicology in vitro : an international journal published in association with BIBRA [Toxicol In Vitro] 2024 Apr; Vol. 96, pp. 105786. Date of Electronic Publication: 2024 Feb 01.
DOI: 10.1016/j.tiv.2024.105786
Abstrakt: Gliomas are among the most common primary malignant brain tumors. Despite advances in cancer treatment, survival is very low, so the discovery of new therapeutic agents is essential. In this context, indole is an important source for the development of new bioactive molecules. A pharmacological screening of ten indole derivatives was carried out to evaluate the cytotoxic capacity against three tumor cell lines. After pharmacological screening, three compounds were selected, based on their high capacity to reduce cell proliferation, and their IC 50 values were determined. Compound 9 exhibited the highest cytotoxic activity (IC 50  = 0.4 μg/mL) in gliomas (C6 cell line), and were selected for further experiments. C6 cells were treated with compound 9 to evaluate cellular mechanisms such as colony formation and cell migration capacity and morphological alterations. Compound 9 decreased clone formation (0.4 and 0.8 μg/mL), and inhibited migration (0.2-0.8 μg/mL) in C6 cells. Morphological changes in cells treated with the compound 9 were also observed, such as chromatin condensation, and disorganization in cellular stress beams. Indole derivatives had a cytotoxic effect on tumor cells, and compound 9 showed the best anti-proliferative and anti-migratory activity in glioma cells.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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