Bridging bench to body: ex vivo models to understand articular cartilage repair.

Autor: Trengove A; Department of Biomedical Engineering, The Graeme Clark Institute, The University of Melbourne, Victoria, Australia; BioFab3D@ACMD, St Vincent's Hospital Melbourne, Fitzroy, Victoria, Australia., Duchi S; BioFab3D@ACMD, St Vincent's Hospital Melbourne, Fitzroy, Victoria, Australia; Department of Orthopaedic Surgery, St Vincent's Hospital Melbourne, Fitzroy, Victoria, Australia., Onofrillo C; BioFab3D@ACMD, St Vincent's Hospital Melbourne, Fitzroy, Victoria, Australia; Department of Orthopaedic Surgery, St Vincent's Hospital Melbourne, Fitzroy, Victoria, Australia., Sooriyaaratchi D; Department of Biomedical Engineering, The Graeme Clark Institute, The University of Melbourne, Victoria, Australia; BioFab3D@ACMD, St Vincent's Hospital Melbourne, Fitzroy, Victoria, Australia., Di Bella C; BioFab3D@ACMD, St Vincent's Hospital Melbourne, Fitzroy, Victoria, Australia; Department of Orthopaedic Surgery, St Vincent's Hospital Melbourne, Fitzroy, Victoria, Australia; Department of Surgery, The University of Melbourne, Victoria, Australia., O'Connor AJ; Department of Biomedical Engineering, The Graeme Clark Institute, The University of Melbourne, Victoria, Australia; BioFab3D@ACMD, St Vincent's Hospital Melbourne, Fitzroy, Victoria, Australia. Electronic address: a.oconnor@unimelb.edu.au.
Jazyk: angličtina
Zdroj: Current opinion in biotechnology [Curr Opin Biotechnol] 2024 Apr; Vol. 86, pp. 103065. Date of Electronic Publication: 2024 Feb 01.
DOI: 10.1016/j.copbio.2024.103065
Abstrakt: With little to no ability to self-regenerate, human cartilage defects of the knee remain a major clinical challenge. Tissue engineering strategies include delivering specific types of cells and biomaterials to the injured cartilage for restoration of architecture and function. Pre-clinical models to test the efficacy of the therapies come with high costs and ethical issues, and imperfect prediction of performance in humans. Ex vivo models represent an alternative avenue to trial cartilage tissue engineering. Defined as viable explanted cartilage samples, ex vivo models can be cultured with a cell-laden biomaterial or tissue-engineered construct to evaluate cartilage repair. Though human and animal ex vivo models are currently used in the field, there is a need for alternative methods to assess the strength of integration, to increase throughput and manage variability and to optimise and standardise culture conditions, enhancing the utility of these models overall.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 The Authors. Published by Elsevier Ltd.. All rights reserved.)
Databáze: MEDLINE
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