Whole-transcriptome sequencing revealed the role of noncoding RNAs in susceptibility and resistance of Pekin ducks to DHAV-3.

Autor:	Ding D; College of Animal Science and Technology, Northwest A&F University, Yangling 712100, Shaanxi, China., Zhao H; College of Animal Science and Technology, Northwest A&F University, Yangling 712100, Shaanxi, China., Liu Y; College of Animal Science and Technology, Northwest A&F University, Yangling 712100, Shaanxi, China., Li S; College of Animal Science and Technology, Northwest A&F University, Yangling 712100, Shaanxi, China., Wei J; Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China., Yang Y; Beijing General Station of Animal Husbandry, Beijing 100101, China., Wang S; Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China., Xing G; Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China., Hou S; Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China., Wang X; College of Animal Science and Technology, Northwest A&F University, Yangling 712100, Shaanxi, China. Electronic address: xiawang@nwafu.edu.cn., Zhang Y; Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Jazyk:	angličtina
Zdroj:	Poultry science [Poult Sci] 2024 Mar; Vol. 103 (3), pp. 103416. Date of Electronic Publication: 2023 Dec 30.
DOI:	10.1016/j.psj.2023.103416
Abstrakt:	As the most prevalent pathogen of duck viral hepatitis (DVH), duck hepatitis A virus genotype 3 (DHAV-3) has caused huge economic losses to the duck industry in China. Herein, we obtained whole-transcriptome sequencing data of susceptible (S) and resistant (R) Pekin duckling samples at 0 h, 12 h, and 24 h after DHAV-3 infection. We found that DHAV-3 infection induces 5,396 differentially expressed genes (DEGs), 85 differentially expressed miRNAs (DEMs), and 727 differentially expressed lncRNAs (DELs) at 24 hpi in S vs. R ducks, those upregulated genes were enriched in inflammation and cell communications pathways and downregulated genes were related to metabolic processes. Upregulated genes showed high connectivity with the miR-33, miR-193, and miR-11591, and downregulated genes were mainly regulated by miR-2954, miR-125, and miR-146b. With the construction of lncRNA-miRNA-mRNA axis, we further identified a few aberrantly expressed lncRNAs (e.g., MSTRG.36194.1, MSTRG.50601.1, MSTRG.34328.7, and MSTRG.29445.1) that regulate expression of hub genes (e.g., THBD, CLIC2, IL8, ACOX2, GPHN, SMLR1, and HAO1) by sponging those highly connected miRNAs. Altogether, our findings defined a dual role of ncRNAs in immune and metabolic regulation during DHAV-3 infection, suggesting potential new targets for treating DHAV-3 infected ducks. (Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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