BATF and BATF3 deficiency alters CD8+ effector/exhausted T cells balance in skin transplantation.

Autor: Li C; Department of Cardiovascular Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China., Liu Z; Department of Cardiovascular Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China., Wang Z; Department of Cardiovascular Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China., Yim WY; Department of Cardiovascular Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China., Huang Y; Department of Plastic Surgery, Xiangyang Central Hospital, Affiliated Hospital of Hubei University of Arts and Science, 136 Jingzhou Street, Xiangyang, Hubei, China. surgeonhyj@163.com., Chen Y; Department of Cardiovascular Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China. union_yuqi@hust.edu.cn.
Jazyk: angličtina
Zdroj: Molecular medicine (Cambridge, Mass.) [Mol Med] 2024 Jan 31; Vol. 30 (1), pp. 16. Date of Electronic Publication: 2024 Jan 31.
DOI: 10.1186/s10020-024-00792-0
Abstrakt: Background: It is well-established that CD8 + T-cells play a critical role in graft rejection. The basic leucine zipper ATF-like transcription factor (BATF) and BATF3 are transcriptional factors expressed in T lymphocytes. Herein, we investigated the functions of BATF and BATF3 in the differentiation and exhaustion of CD8 + T cells following alloantigen activation.
Methods: Wild-type CD8 + T cells, BATF-deficient (Batf -/- ) CD8 + T cells, and CD8 + T cells deficient in both BATF and BATF3 (Batf -/- Batf3 -/- ) were transferred to B6.Rag1 -/- mice, which received skin allografts from BALB/c mice. Flow cytometry was conducted to investigate the number of CD8 + T cells and the percentage of effector subsets.
Results: BATF expression positively correlated with effector CD8+ T cell differentiation. BATF and BATF3 deficiency promoted skin allograft long-term survival and attenuated the CD8+ T cell allo-response and cytokine secretion. Finally, BATF and BATF3 deficiency prompted the generation of exhausted CD8+ T cells.
Conclusions: Overall, our findings provide preliminary evidence that both BATF and BATF3 deficiency influences the differentiation of effector CD8+ T cells and mediates the exhaustion of CD8 + T cells, prolonging transplant survival. Targeting BATF and BATF3 to inhibit CD8 + T cell function has huge prospects for application as a therapeutic approach to prevent transplant rejection.
(© 2024. The Author(s).)
Databáze: MEDLINE
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