Chagas Disease and Leishmaniasis, Sympatric Zoonoses Present in Human from Rural Communities of Venezuela.

Autor: Ferrer E; Instituto de Investigaciones Biomédicas 'Dr. Francisco J. Triana Alonso' (BIOMED), Universidad de Carabobo Sede Aragua, calle Cecilio Acosta, Urb. La Rinconada, Las Delicias, Maracay, estado Aragua, Venezuela. elizabeth.ferrer@gmail.com.; Departamento de Parasitología, Facultad de Ciencias de la Salud, Universidad de Carabobo Sede Aragua, Maracay, estado Aragua, Venezuela. elizabeth.ferrer@gmail.com., Aguilar CM; Centro de Investigaciones en Enfermedades Tropicales (CIET-UC), Facultad de Ciencias de la Salud, Universidad de Carabobo, San Carlos, Cojedes, Venezuela., Viettri M; Instituto de Investigaciones Biomédicas 'Dr. Francisco J. Triana Alonso' (BIOMED), Universidad de Carabobo Sede Aragua, calle Cecilio Acosta, Urb. La Rinconada, Las Delicias, Maracay, estado Aragua, Venezuela., Torrellas A; Instituto de Investigaciones Biomédicas 'Dr. Francisco J. Triana Alonso' (BIOMED), Universidad de Carabobo Sede Aragua, calle Cecilio Acosta, Urb. La Rinconada, Las Delicias, Maracay, estado Aragua, Venezuela., Lares M; Instituto de Investigaciones Biomédicas 'Dr. Francisco J. Triana Alonso' (BIOMED), Universidad de Carabobo Sede Aragua, calle Cecilio Acosta, Urb. La Rinconada, Las Delicias, Maracay, estado Aragua, Venezuela., Diaz M; Centro de Investigaciones en Enfermedades Tropicales (CIET-UC), Facultad de Ciencias de la Salud, Universidad de Carabobo, San Carlos, Cojedes, Venezuela., Delgado O; Instituto de Medicina Tropical (IMT), Facultad de Medicina, Universidad Central de Venezuela (UCV), Caracas, Venezuela., Feliciangeli MD; Instituto de Investigaciones Biomédicas 'Dr. Francisco J. Triana Alonso' (BIOMED), Universidad de Carabobo Sede Aragua, calle Cecilio Acosta, Urb. La Rinconada, Las Delicias, Maracay, estado Aragua, Venezuela., Herrera L; Instituto de Zoología y Ecología Tropical (IZET), Facultad de Ciencias, Universidad Central de Venezuela (UCV), Caracas, Venezuela.
Jazyk: angličtina
Zdroj: Acta parasitologica [Acta Parasitol] 2024 Mar; Vol. 69 (1), pp. 616-627. Date of Electronic Publication: 2024 Jan 31.
DOI: 10.1007/s11686-023-00786-8
Abstrakt: Purpose: Trypanosoma cruzi and Leishmania spp. coexist in several endemic areas, and there are few studies of Chagas disease and leishmaniasis coinfection worldwide; for this reason, the objective of this work was to determine the Chagas disease and leishmaniasis coinfection in several rural communities co-endemic for these diseases.
Methods: A total of 1107 human samples from six co-endemic rural communities of Cojedes state, Venezuela, were analyzed. Serum samples were evaluated by ELISA, indirect hemagglutination, and indirect immunofluorescence for Chagas disease diagnosis, and individuals were evaluated for leishmaniasis by leishmanin skin test (LST). Approximately, 30% of the individuals were also analyzed by PCR (blood clot samples) for T. cruzi and for Leishmania spp.
Results: The 14.7% of the individuals were positive to Trypanosoma cruzi infection by serology, and 25.8% were positive to Leishmania spp. current or past infection by LST. Among the group with PCR results, 7.8% were positive for T. cruzi, and 9.4% for Leishmania spp. The coinfection T. cruzi/Leishmania spp. was 6.5%. The T. cruzi DTUs of the positive blood clot samples were TcI, revealed using the molecular markers: (i) intergenic region of the miniexon, (ii) D7 divergent domain of the 24Sα rDNA, (iii) size-variable domain of the 18S rDNA, and (iv) hsp60-PCR-RFLP (EcoRV). The Leishmania species identified were L. (Leishmania) mexicana and L. (Viannia) braziliensis.
Conclusion: A high prevalence was found for T. cruzi and Leishmania spp. single and coinfections in almost all communities studied, being these results of relevance for the implementation of control programs in co-endemic areas.
(© 2024. The Author(s), under exclusive licence to Springer Nature Switzerland AG.)
Databáze: MEDLINE
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