Cargo-specific effects of hypoxia on clathrin-mediated trafficking.

Autor: van Belle GJ; Institute of Cardiovascular Physiology, University Medical Center Göttingen, Georg-August University, 37073, Göttingen, Germany., Zieseniss A; Institute of Cardiovascular Physiology, University Medical Center Göttingen, Georg-August University, 37073, Göttingen, Germany., Heidenreich D; Institute of Cardiovascular Physiology, University Medical Center Göttingen, Georg-August University, 37073, Göttingen, Germany., Olmos M; Institute of Cardiovascular Physiology, University Medical Center Göttingen, Georg-August University, 37073, Göttingen, Germany., Zhuikova A; Institute of Cardiovascular Physiology, University Medical Center Göttingen, Georg-August University, 37073, Göttingen, Germany., Möbius W; Department of Neurogenetics, Electron Microscopy, City Campus, Max-Planck-Institute for Multidisciplinary Sciences, 37075, Göttingen, Germany., Paul MW; Department of Molecular Genetics, Oncode Institute, Erasmus MC Cancer Institute, Erasmus University Medical Center, Rotterdam, Netherlands., Katschinski DM; Institute of Cardiovascular Physiology, University Medical Center Göttingen, Georg-August University, 37073, Göttingen, Germany. doerthe.katschinski@med.uni-goettingen.de.
Jazyk: angličtina
Zdroj: Pflugers Archiv : European journal of physiology [Pflugers Arch] 2024 Sep; Vol. 476 (9), pp. 1399-1410. Date of Electronic Publication: 2024 Jan 31.
DOI: 10.1007/s00424-024-02911-6
Abstrakt: Clathrin-associated trafficking is a major mechanism for intracellular communication, as well as for cells to communicate with the extracellular environment. A decreased oxygen availability termed hypoxia has been described to influence this mechanism in the past. Mostly biochemical studies were applied in these analyses, which miss spatiotemporal information. We have applied live cell microscopy and a newly developed analysis script in combination with a GFP-tagged clathrin-expressing cell line to obtain insight into the dynamics of the effect of hypoxia. Number, mobility and directionality of clathrin-coated vesicles were analysed in non-stimulated cells as well as after stimulation with epidermal growth factor (EGF) or transferrin in normoxic and hypoxic conditions. These data reveal cargo-specific effects, which would not be observable with biochemical methods or with fixed cells and add to the understanding of cell physiology in hypoxia. The stimulus-dependent consequences were also reflected in the final cellular output, i.e. decreased EGF signaling and in contrast increased iron uptake in hypoxia.
(© 2024. The Author(s).)
Databáze: MEDLINE
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