Antimicrobial activity of hydralazine against methicillin-resistant and methicillin-susceptible Staphylococcus aureus .

Autor:	Stefany Aires do Nascimento FB; School of Pharmacy, Laboratory of Bioprospection of Antimicrobial Molecules (LABIMAN), Federal University of Ceará, Fortaleza, CE, 60430-372, Brazil.; Drug Research & Development Center, Federal University of Ceará, Fortaleza, CE, 60430-275, Brazil., do Amaral Valente Sá LG; School of Pharmacy, Laboratory of Bioprospection of Antimicrobial Molecules (LABIMAN), Federal University of Ceará, Fortaleza, CE, 60430-372, Brazil.; Drug Research & Development Center, Federal University of Ceará, Fortaleza, CE, 60430-275, Brazil.; Christus University Center (UNICHRISTUS), Fortaleza, CE, 60190-180, Brazil., de Andrade Neto JB; School of Pharmacy, Laboratory of Bioprospection of Antimicrobial Molecules (LABIMAN), Federal University of Ceará, Fortaleza, CE, 60430-372, Brazil.; Drug Research & Development Center, Federal University of Ceará, Fortaleza, CE, 60430-275, Brazil.; Christus University Center (UNICHRISTUS), Fortaleza, CE, 60190-180, Brazil., da Silva LJ; School of Pharmacy, Laboratory of Bioprospection of Antimicrobial Molecules (LABIMAN), Federal University of Ceará, Fortaleza, CE, 60430-372, Brazil.; Drug Research & Development Center, Federal University of Ceará, Fortaleza, CE, 60430-275, Brazil., Rodrigues DS; School of Pharmacy, Laboratory of Bioprospection of Antimicrobial Molecules (LABIMAN), Federal University of Ceará, Fortaleza, CE, 60430-372, Brazil.; Drug Research & Development Center, Federal University of Ceará, Fortaleza, CE, 60430-275, Brazil., de Farias Cabral VP; School of Pharmacy, Laboratory of Bioprospection of Antimicrobial Molecules (LABIMAN), Federal University of Ceará, Fortaleza, CE, 60430-372, Brazil.; Drug Research & Development Center, Federal University of Ceará, Fortaleza, CE, 60430-275, Brazil., Barbosa AD; School of Pharmacy, Laboratory of Bioprospection of Antimicrobial Molecules (LABIMAN), Federal University of Ceará, Fortaleza, CE, 60430-372, Brazil.; Drug Research & Development Center, Federal University of Ceará, Fortaleza, CE, 60430-275, Brazil., Almeida Moreira LE; School of Pharmacy, Laboratory of Bioprospection of Antimicrobial Molecules (LABIMAN), Federal University of Ceará, Fortaleza, CE, 60430-372, Brazil.; Drug Research & Development Center, Federal University of Ceará, Fortaleza, CE, 60430-275, Brazil., Braga Vasconcelos CR; School of Pharmacy, Laboratory of Bioprospection of Antimicrobial Molecules (LABIMAN), Federal University of Ceará, Fortaleza, CE, 60430-372, Brazil.; Drug Research & Development Center, Federal University of Ceará, Fortaleza, CE, 60430-275, Brazil., Cavalcanti BC; Drug Research & Development Center, Federal University of Ceará, Fortaleza, CE, 60430-275, Brazil.; Department of Physiology & Pharmacology, Federal University of Ceará, Fortaleza, CE, 60430-275, Brazil., França Rios ME; Drug Research & Development Center, Federal University of Ceará, Fortaleza, CE, 60430-275, Brazil.; Department of Physiology & Pharmacology, Federal University of Ceará, Fortaleza, CE, 60430-275, Brazil., Silva J; Department of Chemistry, Group of Theoretical Chemistry & Electrochemistry (GQTE), State University of Ceará, Limoeiro do Norte, Ceará, 62930-000, Brazil., Marinho ES; Department of Chemistry, Group of Theoretical Chemistry & Electrochemistry (GQTE), State University of Ceará, Limoeiro do Norte, Ceará, 62930-000, Brazil., Dos Santos HS; Science & Technology Centre, Course of Chemistry, State University Vale do Acaraú, Sobral, CE, 62010-560, Brazil., de Mesquita JR; St. Joseph Hospital for Infectious Diseases, Fortaleza, CE, 60455-610, Brazil., Pinto Lobo MD; Department of Biology, Federal University of Ceará, Fortaleza, CE, 60440-900, Brazil., de Moraes MO; Drug Research & Development Center, Federal University of Ceará, Fortaleza, CE, 60430-275, Brazil.; Department of Physiology & Pharmacology, Federal University of Ceará, Fortaleza, CE, 60430-275, Brazil., Nobre Júnior HV; School of Pharmacy, Laboratory of Bioprospection of Antimicrobial Molecules (LABIMAN), Federal University of Ceará, Fortaleza, CE, 60430-372, Brazil.; Drug Research & Development Center, Federal University of Ceará, Fortaleza, CE, 60430-275, Brazil., da Silva CR; School of Pharmacy, Laboratory of Bioprospection of Antimicrobial Molecules (LABIMAN), Federal University of Ceará, Fortaleza, CE, 60430-372, Brazil.; Drug Research & Development Center, Federal University of Ceará, Fortaleza, CE, 60430-275, Brazil.
Jazyk:	angličtina
Zdroj:	Future microbiology [Future Microbiol] 2024 Jan; Vol. 19, pp. 91-106. Date of Electronic Publication: 2024 Jan 31.
DOI:	10.2217/fmb-2023-0160
Abstrakt:	Background: Staphylococcus aureus is a human pathogen responsible for high mortality rates. The development of new antimicrobials is urgent. Materials & methods: The authors evaluated the activity of hydralazine along with its synergism with other drugs and action on biofilms. With regard to action mechanisms, the authors evaluated cell viability, DNA damage and molecular docking. Results: MIC and minimum bactericidal concentration values ranged from 128 to 2048 μg/ml. There was synergism with oxacillin (50%) and vancomycin (25%). Hydralazine reduced the viability of biofilms by 50%. After exposure to hydralazine 2× MIC, 58.78% of the cells were unviable, 62.07% were TUNEL positive and 27.03% presented damage in the comet assay (p < 0.05). Hydralazine showed affinity for DNA gyrase and TyrRS. Conclusion: Hydralazine is a potential antibacterial.
Databáze:	MEDLINE
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