| Autor: |
Kalkeri R; Novavax, Inc., Gaithersburg, MD, USA., Zhu M; Novavax, Inc., Gaithersburg, MD, USA., Cloney-Clark S; Novavax, Inc., Gaithersburg, MD, USA., Plested JS; Novavax, Inc., Gaithersburg, MD, USA., Parekh A; Novavax, Inc., Gaithersburg, MD, USA., Gorinson D; Novavax, Inc., Gaithersburg, MD, USA., Cai R; Novavax, Inc., Gaithersburg, MD, USA., Mahato S; Novavax, Inc., Gaithersburg, MD, USA., Ramanathan P; Department of Microbiology and Immunology, University of Melbourne, at the Peter Doherty Institute for Infection and Immunity, Victoria, 3000, Australia., Aurelia LC; Department of Microbiology and Immunology, University of Melbourne, at the Peter Doherty Institute for Infection and Immunity, Victoria, 3000, Australia., Selva KJ; Department of Microbiology and Immunology, University of Melbourne, at the Peter Doherty Institute for Infection and Immunity, Victoria, 3000, Australia., Marchese AM; Novavax, Inc., Gaithersburg, MD, USA., Fries L; Novavax, Inc., Gaithersburg, MD, USA., Chung AW; Department of Microbiology and Immunology, University of Melbourne, at the Peter Doherty Institute for Infection and Immunity, Victoria, 3000, Australia., Dunkle LM; Novavax, Inc., Gaithersburg, MD, USA. |
| Abstrakt: |
Repeated mRNA SARS-CoV-2 vaccination has been associated with increases in the proportion of IgG4 in spike-specific antibody responses and concurrent reductions in Fcγ-mediated effector functions that may limit control of viral infection. Here, we assessed anti-Spike total IgG, IgG1, IgG2, IgG3 and IgG4, and surrogate markers for antibody-dependent cellular phagocytosis (ADCP, FcγRIIa binding), antibody-dependent cellular cytotoxicity (ADCC, FcγRIIIa binding), and antibody-dependent complement deposition (ADCD, C1q binding) associated with repeated SARS-CoV-2 vaccination with NVX-CoV2373 (Novavax Inc., Gaithersburg, MD). The NVX-CoV2373 protein vaccine did not induce notable increases in spike-specific IgG4 or negatively impact surrogates for Fcγ effector responses. Conversely, repeated NVX-CoV2373 vaccination uniquely enhanced IgG3 responses which are known to exhibit strong affinity for FcγRIIIa and have previously been linked to potent neutralization of SARS-CoV-2. Subsequent investigations will help to understand the immunological diversity generated by different SARS-CoV-2 vaccine types and have the potential to reshape public health strategies. |
