Quantitative assessment of transforming growth factor-β1 release from dentin matrix upon conditioning with ethylene diamine tetra-acetate, doxycycline hydrochloride, and propolis: An in vitro study.

Autor:	Preetham HS; Department of Conservative Dentistry and Endodontics, Government Dental College and Research Institute, Bengaluru, Karnataka, India., Kumar NK; Department of Conservative Dentistry and Endodontics, Government Dental College and Research Institute, Bengaluru, Karnataka, India., Brigit B; Department of Conservative Dentistry and Endodontics, Government Dental College and Research Institute, Bengaluru, Karnataka, India., Swathisha A; Department of Conservative Dentistry and Endodontics, Government Dental College and Research Institute, Bengaluru, Karnataka, India., Shylaja V; Department of Conservative Dentistry and Endodontics, Government Dental College and Research Institute, Bengaluru, Karnataka, India.
Jazyk:	angličtina
Zdroj:	Journal of conservative dentistry and endodontics [J Conserv Dent Endod] 2023 Sep-Oct; Vol. 26 (5), pp. 564-568. Date of Electronic Publication: 2023 Sep 16.
DOI:	10.4103/JCDE.JCDE_16_23
Abstrakt:	Background: Transforming growth factor-beta 1 (TGF-β1) is a key morphogen in regenerative endodontics that plays a central role in regulating cellular functions. Various chelating agents have been shown to release this growth factor upon conditioning. The objective of the study was to evaluate TGF-β1 release from the dentin matrix upon conditioning with ethylene diamine tetra-acetate (EDTA), doxycycline hydrochloride, and propolis. Materials and Methods: Forty-two human 3 rd molar teeth were collected and coronal portion of the teeth was sectioned to obtain dentin blocks with dimensions 2 mm × 2 mm × 2 mm. The blocks were then randomly divided into three groups depending on the conditioning agent used; Group 1: 17% EDTA, Group 2: doxycycline hydrochloride (100 mg/mL), and Group 3: propolis (250 μg/mL). Conditioned blocks were placed in 0.5 mL of phosphate buffered saline and incubated for 1 week for quantification. Results: Highest TGF-β1 release was noted for propolis (0.21 ng/mL), followed by doxycycline hydrochloride (0.18 ng/mL) and 17% EDTA (0.14 ng/mL). Conclusion: Doxycycline hydrochloride and propolis significantly enhanced the release of TGF-β1 from the dentin matrix compared to EDTA ( P < 0.05). No significant difference was observed between doxycycline hydrochloride and propolis ( P > 0.05). Doxycycline and propolis can be used as effective alternatives to EDTA during regenerative endodontic procedures. Competing Interests: There are no conflicts of interest. (Copyright: © 2023 Journal of Conservative Dentistry and Endodontics.)
Databáze:	MEDLINE
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