Monitoring of hepatitis E virus in wastewater can identify clinically relevant variants.

Autor: Rau F; Medical Faculty, Department for Molecular and Medical Virology, Ruhr University Bochum, Bochum, Germany., Elsner C; Institute for Virology, University Hospital Essen, University of Duisburg-Essen, Essen, Germany., Meister TL; Medical Faculty, Department for Molecular and Medical Virology, Ruhr University Bochum, Bochum, Germany.; Institute for Infection Research and Vaccine Development, University Medical Centre Hamburg Eppendorf, Hamburg, Germany.; Department for Clinical Immunology of Infectious Diseases, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany.; German Centre for Infection Research (DZIF), Partner siteHamburg-Lübeck-Borstel-Riems, Hamburg, Germany., Gömer A; Medical Faculty, Department for Molecular and Medical Virology, Ruhr University Bochum, Bochum, Germany., Kallies R; Department of Environmental Microbiology, Helmholtz Centre for Environmental Research-UFZ, Leipzig, Germany., Dittmer U; Institute for Virology, University Hospital Essen, University of Duisburg-Essen, Essen, Germany., Steinmann E; Medical Faculty, Department for Molecular and Medical Virology, Ruhr University Bochum, Bochum, Germany.; German Centre for Infection Research (DZIF), External Partner Site, Bochum, Germany., Todt D; Medical Faculty, Department for Molecular and Medical Virology, Ruhr University Bochum, Bochum, Germany.; European Virus Bioinformatics Center (EVBC), Jena, Germany.
Jazyk: angličtina
Zdroj: Liver international : official journal of the International Association for the Study of the Liver [Liver Int] 2024 Mar; Vol. 44 (3), pp. 637-643. Date of Electronic Publication: 2024 Jan 30.
DOI: 10.1111/liv.15842
Abstrakt: Hepatitis E virus (HEV) is prevalent worldwide and can cause persistent infection with severe morbidity. Antiviral treatment approaches can lead to the emergence of viral variants encoding escape mutations that may impede viral clearance. The frequency of these variants remains unknown in the human population as well as environment due to limited comprehensive data on HEV diversity. In this study, we investigated the HEV prevalence and diversity of circulating variants in environmental samples, that is, wastewater and rivers from North-Rhine Westphalia, Germany. HEV prevalence could be determined with 73% of samples tested positive for viral RNA via qRT-PCR. Using high-throughput sequencing, we were able to assess the overall genetic diversity in these samples and identified the presence of clinically relevant variants associated with drug resistance. In summary, monitoring variants from environmental samples could provide valuable insights into estimating HEV prevalence and identifying circulating variants that can impact treatment outcome.
(© 2024 The Authors. Liver International published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
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