Chromosome-level assembly and analysis of Camelina neglecta: a novel diploid model for Camelina biotechnology research.

Autor: Wang S; National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, China., Blume RY; Institute of Food Biotechnology and Genomics, National Academy of Sciences of Ukraine, Kiev, Ukraine., Zhou ZW; National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, China., Lu S; National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, China.; Hubei Hongshan Laboratory, Wuhan, China., Nazarenus TJ; Center for Plant Science Innovation and Department of Biochemistry, University of Nebraska-Lincoln, Lincoln, USA., Blume YB; Institute of Food Biotechnology and Genomics, National Academy of Sciences of Ukraine, Kiev, Ukraine., Xie W; National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, China.; Hubei Hongshan Laboratory, Wuhan, China., Cahoon EB; Center for Plant Science Innovation and Department of Biochemistry, University of Nebraska-Lincoln, Lincoln, USA. ecahoon2@unl.edu., Chen LL; College of Life Science and Technology, Guangxi University, Nanning, China. llchen@gxu.edu.cn., Guo L; National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, China. guoliang@mail.hzau.edu.cn.; Hubei Hongshan Laboratory, Wuhan, China. guoliang@mail.hzau.edu.cn.; Shenzhen Institute of Nutrition and Health, Huazhong Agricultural University, Wuhan, China. guoliang@mail.hzau.edu.cn.; Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, China. guoliang@mail.hzau.edu.cn.
Jazyk: angličtina
Zdroj: Biotechnology for biofuels and bioproducts [Biotechnol Biofuels Bioprod] 2024 Jan 31; Vol. 17 (1), pp. 17. Date of Electronic Publication: 2024 Jan 31.
DOI: 10.1186/s13068-024-02466-9
Abstrakt: Camelina neglecta is a new diploid Brassicaceae species, which has great research value because of its close relationship with the hexaploid oilseed crop Camelina sativa. Here, we report a chromosome-level assembly of C. neglecta with a total length of 210 Mb. By adopting PacBio sequencing and Hi-C technology, the C. neglecta genome was assembled into 6 chromosomes with scaffold N50 of 29.62 Mb. C. neglecta has undergone the whole-genome triplication (γ) shared among eudicots and two whole-genome duplications (α and β) shared by crucifers, but it has not undergone a specific whole-genome duplication event. By synteny analysis between C. neglecta and C. sativa, we successfully used the method of calculating Ks to distinguish the three subgenomes of C. sativa and determined that C. neglecta was closest to the first subgenome (SG1) of C. sativa. Further, transcriptomic analysis revealed the key genes associated with seed oil biosynthesis and its transcriptional regulation, including SAD, FAD2, FAD3, FAE1, ABI3, WRI1 and FUS3 displaying high expression levels in C. neglecta seeds. The high representability of C. neglecta as a model species for Camelina-based biotechnology research has been demonstrated for the first time. In particular, floral Agrobacterium tumefaciens infiltration-based transformation of C. neglecta, leading to overexpression of CvLPAT2, CpDGAT1 and CvFatB1 transgenes, was demonstrated for medium-chain fatty acid accumulation in C. neglecta seed oil. This study provides an important genomic resource and establishes C. neglecta as a new model for oilseed biotechnology research.
(© 2024. The Author(s).)
Databáze: MEDLINE
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