Profiling of testis-specific or testis-predominant genes expressed in mouse male germ cell lines GC-1 and GC-2.

Autor: Choi S; School of Life Sciences, Gwangju Institute of Science and Technology, Gwangju, 61005, Korea.; R&D Center, KYNOGEN Corp, Suwon, Gyeonggi-do, Korea., Hong SH; School of Life Sciences, Gwangju Institute of Science and Technology, Gwangju, 61005, Korea.; RNA Therapeutics Institute, University of Massachusetts Chan Medical School, Worcester, MA, USA., Han G; School of Life Sciences, Gwangju Institute of Science and Technology, Gwangju, 61005, Korea., Cho C; School of Life Sciences, Gwangju Institute of Science and Technology, Gwangju, 61005, Korea. choch@gist.ac.kr.
Jazyk: angličtina
Zdroj: Genes & genomics [Genes Genomics] 2024 Mar; Vol. 46 (3), pp. 279-287. Date of Electronic Publication: 2024 Jan 30.
DOI: 10.1007/s13258-023-01488-1
Abstrakt: Background: Spermatogenesis is a tightly organized process that utilizes an intrinsic genetic program composed of germ cell-specific genes. Although mouse germ cell-related cell lines are available, few germ cell-specific genes have been comprehensively identified in such cell lines.
Objective: We aimed to profile gene expression in the male mouse germ cell-related cell lines, GC-1 and GC-2, characterize their transcriptomic nature, and identify potential testis- or germ cell-specific or -predominant genes expressed in these cell lines.
Methods: We performed profiling analysis of genes transcribed in the mouse germ cell-related cell lines, GC-1 and GC-2, using our previous microarray data together with public transcriptome information. We analyzed the expression of a number of the cell line genes predicted to be preferentially expressed in testis by RT-PCR.
Results: We found that most testis-specific or -predominant mRNAs are not expressed in GC-1 and GC-2 cells, implying that these cell lines have lost their testis- or germ cell-specific genetic characteristics. RT-PCR analysis of genes predicted to be expressed in the cell lines with preferential testicular expression showed the testis-specific or -predominant expression of nine genes and verified four of them as being expressed in the germ cell lines. Among them, only cyclin-dependent kinase inhibitor 3 genes (Cdkn3) showed testis and germ cell specificity.
Conclusion: Our study provides extensive transcriptomic information to shed light on the limited testicular characteristics of the mouse male germ cell-derived cell lines, GC-1 and GC-2, and offers a list of germ cell line genes with testicular preference.
(© 2024. The Author(s) under exclusive licence to The Genetics Society of Korea.)
Databáze: MEDLINE
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