Plant pathogenic bacterium Ralstonia solanacearum can rapidly evolve tolerance to antimicrobials produced by Pseudomonas biocontrol bacteria.

Autor:	Clough SE; Department of Biology, University of York, York, United Kingdom.; Department of Biosciences, Durham University, Durham, United Kingdom.; Department of Chemistry, Durham University, Durham, United Kingdom., Elphinstone JG; Fera Science Ltd, National Agri-Food Innovation Campus, York, United Kingdom., Friman VP; Department of Biology, University of York, York, United Kingdom.; Department of Microbiology, University of Helsinki, Helsinki, Finland.
Jazyk:	angličtina
Zdroj:	Journal of evolutionary biology [J Evol Biol] 2024 Feb 14; Vol. 37 (2), pp. 225-237.
DOI:	10.1093/jeb/voae002
Abstrakt:	Soil-borne plant pathogens significantly threaten crop production due to lack of effective control methods. One alternative to traditional agrochemicals is microbial biocontrol, where pathogen growth is suppressed by naturally occurring bacteria that produce antimicrobial chemicals. However, it is still unclear if pathogenic bacteria can evolve tolerance to biocontrol antimicrobials and if this could constrain the long-term efficacy of biocontrol strategies. Here we used an in vitro experimental evolution approach to investigate if the phytopathogenic Ralstonia solanacearum bacterium, which causes bacterial wilt disease, can evolve tolerance to antimicrobials produced by Pseudomonas bacteria. We further asked if tolerance was specific to pairs of R. solanacearum and Pseudomonas strains and certain antimicrobial compounds produced by Pseudomonas. We found that while all R. solanacearum strains could initially be inhibited by Pseudomonas strains, this inhibition decreased following successive subculturing with or without Pseudomonas supernatants. Using separate tolerance assays, we show that the majority of R. solanacearum strains evolved increased tolerance to multiple Pseudomonas strains. Mechanistically, evolved tolerance was most likely linked to reduced susceptibility to orfamide lipopeptide antimicrobials secreted by Pseudomonas strains in our experimental conditions. Some levels of tolerance also evolved in the control treatments, which was likely correlated response due to adaptations to the culture media. Together, these results suggest that plant-pathogenic bacteria can rapidly evolve increased tolerance to bacterial antimicrobial compounds, which could reduce the long-term efficacy of microbial biocontrol. (© The Author(s) 2024. Published by Oxford University Press on behalf of the European Society of Evolutionary Biology.)
Databáze:	MEDLINE
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