| Autor: |
Rooney LM; Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, UK., Dupuy LX; The James Hutton Institute, Invergowrie, Dundee, DD2 5DA, UK.; Present address: Department of Conservation of Natural Resources, Neiker, Basque Institute for Agricultural Research and Development, Derio, Spain.; Present address: Ikerbasque, Basque Foundation for Science, Bilbao, Spain., Hoskisson PA; Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, UK., McConnell G; Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, UK. |
| Abstrakt: |
We have developed a tuneable workflow for the study of soil microbes in an imitative 3D soil environment that is compatible with routine and advanced optical imaging, is chemically customisable, and is reliably refractive index matched based on the carbon catabolism of the study organism. We demonstrate our transparent soil pipeline with two representative soil organisms, Bacillus subtilis and Streptomyces coelicolor , and visualise their colonisation behaviours using fluorescence microscopy and mesoscopy. This spatially structured, 3D approach to microbial culture has the potential to further study the behaviour of bacteria in conditions matching their native environment and could be expanded to study microbial interactions, such as competition and warfare. |
