Genome-wide association studies for earliness, MYMIV resistance, and other associated traits in mungbean ( Vigna radiata L. Wilczek) using genotyping by sequencing approach.

Autor:	Kohli M; Amity Institute of Biotechnology, Amity University, Noida, Uttar Pradesh, India.; Genetics, Indian Agricultural Research Institute, Delhi, Delhi, India., Bansal H; Amity Institute of Biotechnology, Amity University, Noida, Uttar Pradesh, India., Mishra GP; Genetics, Indian Agricultural Research Institute, Delhi, Delhi, India., Dikshit HK; Genetics, Indian Agricultural Research Institute, Delhi, Delhi, India., Reddappa SB; Genetics, Indian Agricultural Research Institute, Delhi, Delhi, India., Roy A; Division of Plant Pathology, Indian Agricultural Research Institute, New Delhi, Delhi, India., Sinha SK; Biotechnology, National Institute of Plant Biotechnology, New Delhi, Delhi, India., Shivaprasad KM; Genetics, Indian Agricultural Research Institute, Delhi, Delhi, India., Kumari N; Genetics, Indian Agricultural Research Institute, Delhi, Delhi, India., Kumar A; Division of Seed Science and Technology, Indian Agricultural Research Institute, New Delhi, Delhi, India., Kumar RR; Biochemistry, Indian Agricultural Research Institute, New Delhi, Delhi, India., Nair RM; World Vegetable Center, South Asia, ICRISAT Campus, Patancheru, Hyderabad, Andhra Pradesh, India., Aski M; Genetics, Indian Agricultural Research Institute, Delhi, Delhi, India.
Jazyk:	angličtina
Zdroj:	PeerJ [PeerJ] 2024 Jan 26; Vol. 12, pp. e16653. Date of Electronic Publication: 2024 Jan 26 (Print Publication: 2024).
DOI:	10.7717/peerj.16653
Abstrakt:	Yellow mosaic disease (YMD) remains a major constraint in mungbean ( Vigna radiata (L.)) production; while short-duration genotypes offer multiple crop cycles per year and help in escaping terminal heat stress, especially during summer cultivation. A comprehensive genotyping by sequencing (GBS)-based genome-wide association studies (GWAS) analysis was conducted using 132 diverse mungbean genotypes for traits like flowering time, YMD resistance, soil plant analysis development (SPAD) value, trichome density, and leaf area. The frequency distribution revealed a wide range of values for all the traits. GBS studies identified 31,953 high-quality single nucleotide polymorphism (SNPs) across all 11 mungbean chromosomes and were used for GWAS. Structure analysis revealed the presence of two genetically distinct populations based on ΔK. The linkage disequilibrium (LD) varied throughout the chromosomes and at r 2 = 0.2, the mean LD decay was estimated as 39.59 kb. Two statistical models, mixed linear model (MLM) and Bayesian-information and Linkage-disequilibrium Iteratively Nested Keyway (BLINK) identified 44 shared SNPs linked with various candidate genes. Notable candidate genes identified include FPA for flowering time (VRADI10G01470; chr. 10), TIR-NBS-LRR for mungbean yellow mosaic India virus (MYMIV) resistance (VRADI09G06940; chr. 9), E3 ubiquitin-protein ligase RIE1 for SPAD value (VRADI07G28100; chr. 11), WRKY family transcription factor for leaf area (VRADI03G06560; chr. 3), and LOB domain-containing protein 21 for trichomes (VRADI06G04290; chr. 6). In-silico validation of candidate genes was done through digital gene expression analysis using Arabidopsis orthologous (compared with Vigna radiata genome). The findings provided valuable insight for marker-assisted breeding aiming for the development of YMD-resistant and early-maturing mungbean varieties. Competing Interests: The authors declare there are no competing interests. (©2024 Kohli et al.)
Databáze:	MEDLINE
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